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Subgenomic RNA7 is Transcribed with Different Leader-Body Junction Sites in PRRSV (Strain VR2332) Infection of CL2621 Cells

  • Kay S. Faaberg
  • Margaret R. Elam
  • Chris J. Nelsen
  • Michael P. Murtaugh
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV), like all members of the order Nidoviridae, is expressed in the infected cell as a nested set of subgenomic (sg) RNAs with a common 5’-leader sequence. We have determined that the 5’-leader sequence for the US prototype strain (VR2332, Collins, et al., 1992) is distinct from the European prototype strain [Lelystad (LV); Wensvoort, et al., 1991, Meulenberg et al., 1993a], yet these two strains use almost the same sequence for downstream sites of 5’-leader-body junction formation. Analysis of VR2332 genomic sequence identified several potential 5’-leader-body junction sequences upstream of open reading frame (ORF) 7, coding for the nucleocapsid protein, that could be used for generation of VR2332 sgRNA7 transcripts. Sequence determinations of RT-PCR-generated cDNA clones of sgRNA7 identified two species of RNA7 transcripts in infected cells, one utilizing a leader-body junction sequence (AUAACC) 123 nucleotides upstream of the AUG start site and one utilizing a sequence (UAAACC) 9 nucleotides upstream of the AUG start site for ORF7 translation.

Keywords

Leader Sequence CL2621 Cell Equine Arteritis Virus Lelystad Virus North American Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Kay S. Faaberg
    • 1
  • Margaret R. Elam
    • 1
  • Chris J. Nelsen
    • 1
  • Michael P. Murtaugh
    • 1
  1. 1.Department of Veterinary PathoBiologyUniversity of MinnesotaSt. PaulUSA

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