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Rescue of IBV D-RNA by Heterologous Helper Virus Strains

  • Kathleen Stirrups
  • Kathleen Shaw
  • Sharon Evans
  • Kevin Dalton
  • David Cavanagh
  • Paul Britton
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

Coronavirus defective RNA (D-RNA) vectors could be developed to deliver selected genes for the production of recombinant coronavirus vaccines. An IBV D-RNA, CD-61, derived from a naturally occurring IBV Beaudette D-RNA, CD-91, is being developed as a D-RNA vector for IBV In order to use CD-61 as a vector it will require rescue by heterologous strains in addition to Beaudette. Rescue will be determined by recognition of replication and packaging signals within the D-RNA by the helper virus. The 5’ and 3’ UTRs are believed to contain sequences involved in replication and transcription. The 5’ and 3’ UTRs of six strains of IBV have been sequenced and experiments performed using six strains of helper virus for rescue of CD-61 to determine whether rescue correlates with sequence conservation within the 5’ and 3’ UTRs. Results indicate that all strains of helper virus rescued the D-RNA to varying degrees. Sequence comparisons show a high degree of sequence identity in the UTRs, but enough strain differences exist to be used as markers. The 5’ and 3’ UTRs of the D-RNAs rescued by the heterologous strains were also sequenced and leader switching between the helper virus and the Beaudette leader on the D-RNAs was observed.

Keywords

Infectious Bronchitis Virus Helper Virus Packaging Signal Subsequent Sequence Analysis Heterologous Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Boursnell, M.E.G., Brown, T.D.K., Foulds, I.J., Green, P.F., Tomley, F.M. and Binns, M.M., 1987, Completion of the sequence of the genome of the coronavirus infectious bronchitis virus, J. Gen. Virol. 68: 57–77.PubMedCrossRefGoogle Scholar
  2. Chang, R.-Y., Krishnan, R. and Brian, D.A., 1996, The UCUAAAC Promoter motif is not required for high efficiency leader recombination in bovine coronavirus defective interfering RNA, J. Virol. 70: 2720–2729.PubMedGoogle Scholar
  3. Kottier, S.A., Cavanagh, D. and Britton, P., 1995, Experimental evidence of recombination in coronavirus infectious bronchitis virus, Virology 213: 569–580.PubMedCrossRefGoogle Scholar
  4. Lai, M.M.C., 1990, Coronavirus: Organization, replication and expression of genome, Ann. Rev. Microbiol. 44: 303–333.CrossRefGoogle Scholar
  5. Makino, S. and Lai M.M.C., 1989, High frequency leader switching during coronavirus defective interfering RNA replication, J. Virol. 63: 5285–5292.PubMedGoogle Scholar
  6. Penzes, Z., Tibbies, K., Shaw, K., Britton, P., Brown, T.D.K. and Cavanagh, D., 1994, Characterization of a replicating and packaged defective RNA of Avian coronavirus Infectious Bronchitis Virus, Virology. 203: 286–293.PubMedCrossRefGoogle Scholar
  7. Penzes, Z., Wroe, C, Brown, T.D.K., Britton, P. and Cavanagh, D., 1996, Replication and packaging of coronavirus infectious bronchitis virus defective RNAs lacking a long open reading frame, J. Virol. 70: 8660–8668.PubMedGoogle Scholar
  8. Williams, A.K., Wang, L., Sneed, L.W. and Collisson, E.W., 1993, Analysis of a hypervariable region in the 3’ non-coding region of the infectious bronchitis virus genome, Virus Res. 28: 19–27.PubMedCrossRefGoogle Scholar
  9. Zhang, L., Hornberger, F., Spaan, W. and Luytjes, W., 1997, Recombinant genomic RNA of Coronavirus MHV-A59 after coreplication with a DI RNA containing the MHV-RI spike gene, Virology 230: 93–102.PubMedCrossRefGoogle Scholar
  10. Zwaagstra, K. A., Van Der Zeijst, B. A. M. and Kusters J.G., 1991, Rapid detection and identification of avian infectious bronchitis virus, J. Clin. Microbiol. 30: 70–84.Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Kathleen Stirrups
    • 1
  • Kathleen Shaw
    • 1
  • Sharon Evans
    • 1
  • Kevin Dalton
    • 1
  • David Cavanagh
    • 1
  • Paul Britton
    • 1
  1. 1.Division of Molecular Biology, Institute for Animal HealthCompton LaboratoryComptonUK

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