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Sequence Elements Involved in the Rescue of IBV Defective RNA CD-91

  • Kevin Dalton
  • Z. Penzes
  • C. Wroe
  • K. Stirrups
  • S. Evans
  • K. Shaw
  • T. D. K. Brown
  • P. Britton
  • D. Cavanagh
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

Deletion mutagenesis has been used to identify essential regions for rescue of coronavirus defective RNAs (D-RNAs). Using this technique on a cloned IBV D-RNA CD-91, we have identified a region potentially important in its rescue. Comparing the sequence of D-RNAs rescued with those not rescued we have deduced that a 72 base region corresponding to base number 13824 to 13896 in the viral genome is required for rescue. This may be an IBV D-RNA packaging signal or a cis-acting element involved in replication. Further experiments and modification of our techniques will be required to differentiate between the two processes.

Keywords

Deletion Mutant Infectious Bronchitis Virus Essential Region Packaging Signal Deletion Mutagenesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Kevin Dalton
    • 1
  • Z. Penzes
    • 1
  • C. Wroe
    • 2
  • K. Stirrups
    • 1
  • S. Evans
    • 1
  • K. Shaw
    • 1
  • T. D. K. Brown
    • 2
  • P. Britton
    • 1
  • D. Cavanagh
    • 1
  1. 1.IAH ComptonEngland
  2. 2.Division of Virology, Department of PathologyUniversity of CambridgeEngland

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