Studies of Murine Coronavirus DI RNA Replication from Negative-Strand Transcripts

  • S. Banerjee
  • S. Makino
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)


The positive-strand transcripts as well as negative-strand transcripts of mouse hepatitis virus (MHV) defective interfering (DI) RNA, when introduced into MHV-infected cells, re-sulted in DI RNA replication and accumulation. The leader sequence of the majority of DI RNAs that accumulated from the expression of negative-strand DI RNA transcripts with no extra non-MHV nucleotides at the 3’ end switched to that of helper virus, whereas this leader sequence switching did not occur in most of the positive-strand DI RNAs that accumulated from the expressed negative-strand DI RNA transcripts with extra non-MHV nucleotides at the 3’ end. These data demonstrated that the extra 4 nucleotides at the 3’-end of negative-strand DI RNA transcripts affected leader sequence switching on DI RNA, and indicated that the leader switching probably occurred during positive-strand DI RNA synthesis.


Leader Sequence Hepatitis Delta Virus Helper Virus Mouse Hepatitis Virus Defective Interfere 
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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • S. Banerjee
    • 1
  • S. Makino
    • 1
  1. 1.Department of Microbiology, and Institute of Cellular and Molecular BiologyThe University of Texas at AustinAustinUSA

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