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Studies of Murine Coronavirus DI RNA Replication from Negative-Strand Transcripts

  • S. Banerjee
  • S. Makino
Chapter
  • 46 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

The positive-strand transcripts as well as negative-strand transcripts of mouse hepatitis virus (MHV) defective interfering (DI) RNA, when introduced into MHV-infected cells, re-sulted in DI RNA replication and accumulation. The leader sequence of the majority of DI RNAs that accumulated from the expression of negative-strand DI RNA transcripts with no extra non-MHV nucleotides at the 3’ end switched to that of helper virus, whereas this leader sequence switching did not occur in most of the positive-strand DI RNAs that accumulated from the expressed negative-strand DI RNA transcripts with extra non-MHV nucleotides at the 3’ end. These data demonstrated that the extra 4 nucleotides at the 3’-end of negative-strand DI RNA transcripts affected leader sequence switching on DI RNA, and indicated that the leader switching probably occurred during positive-strand DI RNA synthesis.

Keywords

Leader Sequence Hepatitis Delta Virus Helper Virus Mouse Hepatitis Virus Defective Interfere 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • S. Banerjee
    • 1
  • S. Makino
    • 1
  1. 1.Department of Microbiology, and Institute of Cellular and Molecular BiologyThe University of Texas at AustinAustinUSA

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