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Cell Proteins Bind to A 67 Nucleotide Sequence within the 3’ Noncoding Region (NCR) of Simian Hemorrhagic Fever Virus (SFV) Negative-Strand RNA

  • You-Kyung Hwang
  • Margo A. Brinton
Chapter
  • 30 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

The 3’NCR of the SHFV negative-strand RNA [SHFV 3’(-)NCR RNA] is thought to be the initiation site of full-length and possibly also subgenomic positive-strand RNA and so is likely to contain cis-acting signals for viral RNA replication. Cellular and viral proteins may specifically interact with this region to form replication complexes. When in vitro transcribed SHFV 3’(-)NCR RNA was used as a probe in gel mobility shift assays, two RNA-protein complexes were detected with MA 104 S100 cytoplasmic extracts. The specificity of these RNA-protein interactions was demonstrated by competition gel mobil-ity shift assays. Four MA104 proteins (103, 86, 55, and 36 kDa) were detected by UV-in-duced cross-linking assays and three proteins (103, 55, and 36 kDa) were detected by northwestern blotting assays. The binding sites for these proteins were mapped to the re-gion between nucleotides 117 to 184 on the SHFV 3’(-)NCR RNA. Four cellular proteins with identical molecular masses to those of the proteins that bind to the SHFV 3’(-)NCR RNA were detected by the 3’(-) NCR of another arterivirus, LDV-C, suggesting that diver-gent arteriviruses utilize the same set of conserved cell protein domains.

Keywords

Cellular Protein West Nile Virus Cytoplasmic Extract Identical Molecular Mass Simian Hemorrhagic Fever Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • You-Kyung Hwang
    • 1
  • Margo A. Brinton
    • 1
  1. 1.Department of BiologyGeorgia State UniversityAtlantaUSA

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