Neutralization of MHV-A59 by Soluble Recombinant Receptor Glycoproteins

  • Bruce D. Zelus
  • David R. Wessner
  • Gabriela S. Dveksler
  • Kathryn V. Holmes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)


The interaction of viruses with specific receptors is an important determinant of viral tissue tropism and species specificity. Our goals are to understand how mouse hepatitis virus (MHV) recognizes its cellular receptor, MHVR, and how post-binding interactions with this receptor influence viral fusion and entry. Murine cells express a variety of cell surface molecule in the biliary glycoprotein (Bgp) family that are closely related to the MHVR. When these proteins are expressed at high levels in cell culture, they function as MHV receptors. We used a baculovirus expression system to produce soluble recombinant murine Bgp receptors in which the transmembrane and cytoplasmic domains have been re-placed with a six-histidine tag. The soluble glycoproteins were purified to apparent homo-geneity and shown to react with antisera to the native receptor. We compared the virus neutralizing activities of various soluble receptor glycoproteins. Soluble MHVR [sMHVR(1–4)] had 10–20 fold more virus neutralizing activity the soluble protein derived from the Bgplb glycoprotein [sBgplb(1–4)], from MHV-resistant SJL mice. The sMHVR(1–4) glycoprotein was 60–100 fold more active than a truncated receptor mole-cule containing only the first two immunoglobulin-like domains, sMHVR(l,2). The obser-vation that sMHVR lacking domains 3 and 4 neutralizes MHV-A59 very poorly suggests that these domains may influence virus binding or subsequent steps associated with neutralization.


Soluble Receptor Virus Neutralize Tissue Tropism Baculovirus Expression System Mouse Hepatitis Virus 
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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Bruce D. Zelus
    • 1
  • David R. Wessner
    • 2
  • Gabriela S. Dveksler
    • 2
  • Kathryn V. Holmes
    • 1
    • 2
  1. 1.Health Sciences Center Denver, Department of MicrobiologyUniversity of ColoradoDenverUSA
  2. 2.Department of PathologyUniformed Services University of the Health SciencesBethesdaUSA

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