Coronaviruses pp 469-470 | Cite as

Construction of a Recombinant Adenovirus for the Expression of the Glycoprotein S Antigen of Porcine Respiratory Coronavirus

  • P. Callebaut
  • M. Pensaert
  • L. Enjuanes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)


Efforts to develop respiratory and enteric vaccines focus on the efficient delivery of protective antigens to the mucosal lymphoid tissue. One approach is to use recombinant viral vectors, i.e. apathogenic viruses with a tropism for mucosal surfaces and genetically manipulated to express the protective antigens. Adenoviruses, particularly the human adenovirus type 5 (Ad5), are promising candidates for use as vectors.


Recombinant Adenovirus Protective Antigen Transmissible Gastroenteritis Virus Polymerase Chain Reaction Process Recombinant Viral Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Callebaut, P., Debouck, P., and Pensaert, M., 1982, Enzyme linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea. Vet. Microbiol. 7: 295.PubMedCrossRefGoogle Scholar
  2. Correa, I., Gebauer, F., Bullido, M., Suné, C., Baay, M.F.D., Zwaagstra, K.A., Posthumus, W.P.A., Lenstra, J.A., Enjuanes, L., 1990, Localization of antigenic sites of the E2 glycoprotein of transmissible gastroenteritis coronavirus. J. Gen. Viro1.71: 271.CrossRefGoogle Scholar
  3. Graham, F.L., and Prevec, L., 1991, Manipulation of adenovirus vectors. In: “Methods in Molecular Biology” Murray, E.J., ed., Humana Press, 109.Google Scholar
  4. Pensaert, M., Callebaut, P., and Vergote, J., 1986, Isolation of porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet. Quart. 8:257.CrossRefGoogle Scholar
  5. Prevec, L., Campbell, J.B., Christie, B.S., Belbeck, L., and Graham, F.L., 1990, A recombinant human adenovirus vaccine against rabies. J. Infect. Dis. 161: 27.PubMedCrossRefGoogle Scholar
  6. Rasschaert, D., Duarte, M., and Laude, H., 1990, Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions. J. Gen. Virol. 71:2599.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • P. Callebaut
    • 1
  • M. Pensaert
    • 1
  • L. Enjuanes
    • 2
  1. 1.Laboratory of Virology, Faculty of Veterinary MedicineUniversity of GentGentBelgium
  2. 2.Centro de Biologia Molecular (CSIC-UAM), Facultad de CienciasUniversidad AutonomaMadridSpain

Personalised recommendations