Coronaviruses pp 413-418 | Cite as

Coronavirus Induced Encephalomyelitis: An Immunodominant CD4+ - T Cell Site on the Nucleocapsid Protein Contributes to Protection

  • Helmut Wege
  • Andreas Schliephake
  • Heiner Körner
  • Egbert Flory
  • Hanna Wege
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)


In this communication we present clear evidence, that the N-protein of MHV-JHM contains immunodominant CD4+T-cell sites. These sites were recognized by the immune system of virus infected Lewis rats. In previous investigations we have shown, that CD4+T-cell lines with specificity for defined viral proteins can be selected from diseased Lewis rats and mediate protection, if transferred to otherwise lethally infected animals1. To define regions of the N-protein, which are immunodominant for the T-cell response, we employed bacterially expressed N-protein and truncated subfragments of N as an antigen. We demonstrate, that T-cells from MHV-JHM infected, diseased Lewis rats recognized with high prevalence the carboxyterminal subfragment C4-N (95 aa) and to some extent the adjacent C3-N protein. The same results were obtained with T-cells derived from rats immunized with bacterially expressed N-protein or from animals vaccinated by a stable N-protein expressing vaccinia recombinant. Finally, transfer of CD4+ line T-cells to MHV-JHM infected rats specific for C4-N mediated protection against acute disease.


Nucleocapsid Protein Virus Elimination Splenocyte Culture Coronavirus Infection Virus Specific Immune Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Helmut Wege
    • 1
  • Andreas Schliephake
    • 1
  • Heiner Körner
    • 1
  • Egbert Flory
    • 1
  • Hanna Wege
    • 1
  1. 1.Institute of Virology and ImmunobiologyWürzburgGermany

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