Coronaviruses pp 267-272 | Cite as

Expression of MHV-A59 Receptor Glycoproteins in Susceptible and Resistant Strains of Mice

  • G. S. Dveksler
  • A. A. Basile
  • C. B. Cardellichio
  • N. Beauchemin
  • C. W. Dieffenbach
  • K. V. Holmes
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)


Band and Warwick showed that inbred mouse strains differed in their susceptibility to virulent strain of mouse hepatitis virus, MHV-2, and that peritoneal macrophages cultured from these mouse strains reflected their differences in susceptibility to the virus (1). Adult SJL/J mice are highly resistant to MHV-JHM and MHV-A59 (2,12,13). Our laboratory showed that membranes from the liver and intestinal epithelial cells of BALB/c mice could bind MHV-A59 virions in a solid phase assay using undenatured membrane proteins, whereas membranes from SJL/J mice did not bind virus, suggesting that differences in binding of virus to cells from different mouse strains could account for their differences in susceptibility to MHV (3). In virus-overlay protein blot assays of intestinal brush border and liver membrane proteins, MHV-A59 virions bound strongly to a 58 kDa glycoprotein from BALB/c mice, but the virus did not recognize any proteins from SJL/J mouse tissues (3,4). Anti-receptor monoclonal antibody MAb-CC1 that blocked infection of murine cell lines with MHV-A59 recognized the 110–120 kDa and 58 kDa membrane glycoproteins of BALB/c mice, but no proteins from SJL/J membranes (6,7).


Inbred Mouse Strain Mouse Hepatitis Virus Intestinal Brush Border Solid Phase Assay Fourth Domain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • G. S. Dveksler
    • 1
  • A. A. Basile
    • 1
  • C. B. Cardellichio
    • 1
  • N. Beauchemin
    • 2
  • C. W. Dieffenbach
    • 3
  • K. V. Holmes
    • 1
  1. 1.Department of PathologyUniformed Services University of the Health SciencesBethesdaUSA
  2. 2.McGill Cancer CentreMcGill UniversityMontrealCanada
  3. 3.Division of AIDS, NIAIDNational Institutes of HealthBethesdaUSA

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