Expression of MHV-A59 Receptor Glycoproteins in Susceptible and Resistant Strains of Mice
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Band and Warwick showed that inbred mouse strains differed in their susceptibility to virulent strain of mouse hepatitis virus, MHV-2, and that peritoneal macrophages cultured from these mouse strains reflected their differences in susceptibility to the virus (1). Adult SJL/J mice are highly resistant to MHV-JHM and MHV-A59 (2,12,13). Our laboratory showed that membranes from the liver and intestinal epithelial cells of BALB/c mice could bind MHV-A59 virions in a solid phase assay using undenatured membrane proteins, whereas membranes from SJL/J mice did not bind virus, suggesting that differences in binding of virus to cells from different mouse strains could account for their differences in susceptibility to MHV (3). In virus-overlay protein blot assays of intestinal brush border and liver membrane proteins, MHV-A59 virions bound strongly to a 58 kDa glycoprotein from BALB/c mice, but the virus did not recognize any proteins from SJL/J mouse tissues (3,4). Anti-receptor monoclonal antibody MAb-CC1 that blocked infection of murine cell lines with MHV-A59 recognized the 110–120 kDa and 58 kDa membrane glycoproteins of BALB/c mice, but no proteins from SJL/J membranes (6,7).
KeywordsInbred Mouse Strain Mouse Hepatitis Virus Intestinal Brush Border Solid Phase Assay Fourth Domain
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