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Coronaviruses pp 123-128 | Cite as

Analysis of Messenger RNA within Virions of IBV

  • Dave Cavanagh
  • Kathy Shaw
  • Zhao Xiaoyan
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 342)

Abstract

The presence of subgenomic mRNAs (sgRNAs) in virions of infectious bronchitis virus was examined by probing Northern blots of RNA extracted from vinons using as a probe a cDNA of the 3’-terminal nucleocapsid protein (N) gene. The sgRNAs were readily detected even after extensive purification of virions and after RNase A treatment of virions. The molar ratio of gRNA to each sgRNA was in the range 25 to 400 for IBV-M41 and 10 to 30 for IBV-Beaudette. After comparison with the molar ratios of genomic to intracellular viral sgRNAs it was estimated that the efficiency of incorporation of gRNA into virions was approximately 100 to 500-fold greater than for sgRNAs in the case of M41 and 20 to 100-fold for Beaudette, depending on the sgRNA species. It is concluded that sgRNAs can be present within IBV virions. Approximately 1 in 3 Beaudette virions and 1 in 20 M41 particles might contain a single copy of one sgRNA.

Keywords

Infectious Bronchitis Virus Allantoic Fluid Murine Hepatitis Virus Guanidinium Isothiocyanates Bovine Coronavirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Dave Cavanagh
    • 1
  • Kathy Shaw
    • 1
  • Zhao Xiaoyan
    • 1
  1. 1.Division of Molecular BiologyAFRC Institute for Animal Health Compton LaboratoryCompton, NewburyUK

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