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Mutants of tRNA, Ribosomes and mRNA Affecting Frameshifting, Hopping or Stop Codon Read-Through

  • J. F. Atkins
  • K. Herbst
  • M. O’Connor
  • T. M. F. Tuohy
  • R. B. Weiss
  • N. M. Wills
  • R. F. Gesteland
Chapter
  • 247 Downloads

Abstract

Programmed non-standard translation elongation events, frameshifting, hopping and read-through, are important in the decoding of a minority of mRNAs from diverse sources. In this chapter we provide an overview of some of the approaches we are taking to investigate this “recoding” (Gesteland et al., 1992). Also included is an overview of some current studies on mutants which cause frameshifting and read-through, at sites where it does not normally occur i.e., non-programmed events.

Keywords

Murine Leukemia Virus Infectious Bronchitis Virus Mouse Mammary Tumor Virus Anticodon Loop Mature tRNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Brierley, I., Digard, P., and Inglis, S.C., 1989, Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot, Cell 57:537.PubMedCrossRefGoogle Scholar
  2. Brierley, I., Rolley, N.J., Jenner, A.J., and Inglis, S.C., 1991, Mutational analysis of the RNA pseudoknot component of a coronavirus ribosomal frameshifting signal, J. Mol. Biol. 220:889.PubMedCrossRefGoogle Scholar
  3. Chamorro, M., Parkin, N., and Varmus, H.E., 1992, An RNA pseudoknot and an optimal heptameric shift site are required for highly efficient ribosomal frameshifting on a retroviral messenger RNA, Proc. Natl. Acad. Sci. USA 89:713.PubMedCrossRefGoogle Scholar
  4. Dayhuff, T.J., Gesteland, R.F., and Atkins, J.F., 1992, Electrophoresis, autoradiography and electroblotting of peptides: T4 gene 60 hopping, Bio Techniques 13:499.Google Scholar
  5. Felsenstein, K.M., and Goff, S.P., 1992, Mutational analysis of the gag-pol junction of Moloney murine leukemia virus: requirements for expression of the gag-pol fusion protein, J Virol. 66:6601.PubMedGoogle Scholar
  6. Feng, Y.-X., Yuan, H., and Rein, A., and Levin, J.G., 1992, Bipartite signal for readthrough suppression in murine leukemia virus mRNA: an eight-nucleotide purine-rich sequence immediately downstream of the gag termination codon followed by an RNA pseudoknot, J. Virol. 66:5127.PubMedGoogle Scholar
  7. Gesteland, R.F., Weiss, R.B., and Atkins, J.F., 1992, Recoding: Reprogrammed genetic decoding, Science 257:1640.PubMedCrossRefGoogle Scholar
  8. Huang, W.M., Ao, S.Z., Casjens, S., Orlandi, R., Zeikus, R., Weiss, R., Winge, D., and Fang, M., 1988, A persistent untranslated sequence within bacteriophage T4 DNA tópoisomerase gene 60, Science 239:1005.PubMedCrossRefGoogle Scholar
  9. Honigman, A., Wolf, D., Yash, S., Falk, H., and Panet, A., 1991, cis Acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus, Virol. 183:313.CrossRefGoogle Scholar
  10. Moazed, D., and Noller, H.F., 1989, Intermediate states in the movement of transfer RNA in the ribosome, Nature 342:142.PubMedCrossRefGoogle Scholar
  11. Tuohy, T.M.F., Thompson, S., Gesteland, R.F., and Atkins, J.F., 1992, Seven, eight and nine-membered anticodon loop mutants of tRNA2 Arg which cause +1 frameshifting. Tolerance of DHU arm and other secondary mutations, J Mol. Biol. 228:1042.PubMedCrossRefGoogle Scholar
  12. Weiss, R.B., Huang, W-M., and Dunn, D.M., 1990, A nascent peptide is required for ribosomal bypass of the coding gap in bacteriophage T4 gene 60, Cell 62:117.PubMedCrossRefGoogle Scholar
  13. Wills, N.M., Gesteland, R.F., and Atkins, J.F., 1991, Evidence that a downstream pseudoknot is required for translational read-through of the Moloney murine leukemia virus gag stop codon, Proc. Natl. Acad. Sci. USA 88:6991.PubMedCrossRefGoogle Scholar
  14. Yoshinaka, Y., Katoh, I., Copeland, T.D., and Oroszian, S., 1985, Murine leukemia virus protease is encoded by the gag-pol gene and is synthesized through suppression of an amber termination codon, Proc. Natl. Acad. Sci. USA 82:1618.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • J. F. Atkins
    • 1
  • K. Herbst
    • 1
    • 2
  • M. O’Connor
    • 1
    • 3
  • T. M. F. Tuohy
    • 1
  • R. B. Weiss
    • 1
  • N. M. Wills
    • 1
    • 2
  • R. F. Gesteland
    • 1
    • 2
  1. 1.Department of Human GeneticsUniversity of UtahSalt Lake CityUSA
  2. 2.Howard Hughes Medical InstituteUniversity of UtahSalt LakeUSA
  3. 3.Section of BiochemistryBrown University, ProvidenceUSA

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