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First Experimental Evidence of Recombination in Infectious Bronchitis Virus

Recombination in IBV
  • Sanneke A. Kottier
  • David Cavanagh
  • Paul Britton
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

A high frequency of recombination has been shown to occur during replication of the coronavirus mouse hepatitis virus (MHV) in vitro as well as in vivo. Although sequencing of field strains of coronavirus infectious bronchitis virus (IBV) has indicated that IBV strains also undergo recombination, there has been no experimental evidence to support this deduction. To investigate whether recombination occurs in IBV, embryonated eggs were coinfected with IBV-Beaudette and IBV-M41. Potential recombinants were detected by strain-specific polymerase chain reaction (PCR) amplifications, using oligonucleotides corresponding to regions in the 3′ end of the genome. Sequencing of the PCR products confirmed that a number of recombinations had occurred between the two strains.

Keywords

Polymerase Chain Reaction Product Prime Combination Infectious Bronchitis Virus Recombination Site Allantoic Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Sanneke A. Kottier
    • 1
  • David Cavanagh
    • 1
  • Paul Britton
    • 1
  1. 1.Division of Molecular BiologyInstitute for Animal HealthCompton, NewburyUK

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