First Experimental Evidence of Recombination in Infectious Bronchitis Virus

Recombination in IBV
  • Sanneke A. Kottier
  • David Cavanagh
  • Paul Britton
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)


A high frequency of recombination has been shown to occur during replication of the coronavirus mouse hepatitis virus (MHV) in vitro as well as in vivo. Although sequencing of field strains of coronavirus infectious bronchitis virus (IBV) has indicated that IBV strains also undergo recombination, there has been no experimental evidence to support this deduction. To investigate whether recombination occurs in IBV, embryonated eggs were coinfected with IBV-Beaudette and IBV-M41. Potential recombinants were detected by strain-specific polymerase chain reaction (PCR) amplifications, using oligonucleotides corresponding to regions in the 3′ end of the genome. Sequencing of the PCR products confirmed that a number of recombinations had occurred between the two strains.


Polymerase Chain Reaction Product Prime Combination Infectious Bronchitis Virus Recombination Site Allantoic Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Lai, M.M.C. (1992). RNA recombination in Animal and Plant Viruses. Microbiology Reviews 56, 61–79.Google Scholar
  2. 2.
    Baric, R.S., Fu, K., Schaad, M.C. &Stohlman, S.A. (1990). Establishing a genetic-recombination map for murine coronavirus strain A59 complementation groups. Virology 177, 646–656.PubMedCrossRefGoogle Scholar
  3. 3.
    Koetzner, C.A., Parker, M.M., Ricard, C.S., Sturman, L.S. &Masters, P.S. (1992). Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination. Journal of Virology 66, 1841–1848.PubMedGoogle Scholar
  4. 4.
    van der Most, R.G., Heijnen, L., Spaan, W.J.M. &de Groot, R.J. (1992). Homologous RNA recombination allows efficient introduction of site-specific mutations into the genome of coronavirus MHV-A59 via synthetic co-replicating RNAs. Nucleic Acids Research 20, 3375–3381.PubMedCrossRefGoogle Scholar
  5. 5.
    Masters, P.S., Koetzner, C.A., Kerr, C.A. &Heo, Y. (1994). Optimization of targeted RNA recombination and mapping of a novel nucleocapsid gene mutation in the coronavirus mouse hepatitis virus. Journal of Virology 68, 328–337.PubMedGoogle Scholar
  6. 6.
    Kirkegaard, K. &Baltimore, D. (1986). The mechanism of RNA recombination in poliovirus. Cell 47,433–443.PubMedCrossRefGoogle Scholar
  7. 7.
    Makino, S., Keck, J.G., Stohlman, S.A. &Lai, M.M.C. (1986). High frequency RNA recombination of murine coronaviruses. Journal of Virology 57, 729–737.PubMedGoogle Scholar
  8. 8.
    Kusters, J.G., Jager, E.J., Niesters, H.G.M. &van der Zeijst, B.A.M. (1990). Sequence evidence for RNA recombination in field isolates of avian coronavirus infectious bronchitis virus. Vaccine 8, 605–608.within the Massachusetts serotype. Avian Pathology 21, 401–408.CrossRefGoogle Scholar
  9. 9.
    Cavanagh, D., Davis, P.J. &Cook, J.K.A. (1992). Infectious bronchitis virus evidence for recombinationGoogle Scholar
  10. 10.
    Wang, L., Junker, D., Collisson, E.W. (1993) Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology 192, 710–716.PubMedCrossRefGoogle Scholar
  11. 11.
    ll.Boursnell, M.E.G., Brown, T.D.K., Foulds, I.J., Green, P.F., Tomley, F.M. &Binns, M.M. (1987).Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. Journal of General Virology 68, 57–77.PubMedCrossRefGoogle Scholar
  12. 12.
    Spaan, W., Cavanagh, D. &Horzinek, M.C. (1988). Coronaviruses: structure and genome expression.Journal of General Virology 69, 2939–2952.PubMedCrossRefGoogle Scholar
  13. 13.
    Chomczynski, P. &Sacchi, N. (1987). Single step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction. Analytical Biochemistry 162, 156–159.PubMedCrossRefGoogle Scholar
  14. 14.
    Banner, L.R. &Lai, M.M.C. (1991). Random nature of coronavirus RNA recombination in the absence of selection pressure. Virology 185, 441–445.PubMedCrossRefGoogle Scholar
  15. 15.
    Jarvis, T.C. &Kirkegaard, K. (1992). Poliovirus RNA recombination: mechanistic studies in the absence of selection. EMBO Journal 11, 3135–3145.PubMedGoogle Scholar
  16. 16.
    Liao, C.-L. &Lai, M.M.C. (1992). RNA recombination in a coronavirus: recombination between viral genomic RNA and transfected RNA fragments. Journal of Virology 66, 6117–6124.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Sanneke A. Kottier
    • 1
  • David Cavanagh
    • 1
  • Paul Britton
    • 1
  1. 1.Division of Molecular BiologyInstitute for Animal HealthCompton, NewburyUK

Personalised recommendations