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Evidence for a Pseudoknot in the 3′ Untranslated Region of the Bovine Coronavirus Genome

  • G. D. Williams
  • R.-Y. Chang
  • D. A. Brian
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

A potential pseudoknot was found in the 3′ untranslated region of the bovine coronavirus genome beginning 63 nt downstream from the stop codon of the N gene. Mutation analysis of the pseudoknot in a cloned defective interfering RNA indicated that this structural element is necessary for defective interfering RNA replication.

Keywords

Brome Mosaic Virus Large Open Reading Frame Pseudoknotted Structure Bovine Coronavirus Corona Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Chang R.-Y., Hofmann M.A., Sethna P.B., Brian D.A. A cis-acting function for the coronavirus leader in defective interfering RNA replication. J Virol 1994;68:8223–8231.PubMedGoogle Scholar
  2. 2.
    Horton R.M., Cai Z., Ho S.N., Pease L.R. Gene splicing by overlap extension: tailor made genes using the polymerase chain reaction. BioTechniques 1990;8:525–535.CrossRefGoogle Scholar
  3. 3.
    Tinoco I., Borer P.N., Dengler B., Levine M.D., Uhlenbeck O.C., Crothers D.M., Gralla J. Improved estimation of secondary structure in ribonucleic acids. Nature (London) New Biol 1973;246:40–41.Google Scholar
  4. 4.
    ten Dam E., Pleij K., Draper D. Structural and functional aspects of RNA pseudoknots. Biochemistry 1992;31:1665–1676.CrossRefGoogle Scholar
  5. 5.
    Dreher T.W., Hall T.C. Mutational analysis of the sequence and structural requirements in Brome Mosaic Virus RNA fro minus strand promoter activity. J Mol Biol 1988;201:31–40.PubMedCrossRefGoogle Scholar
  6. 6.
    De Groot R.J., Van der Most R.G., Spaan W.J.M. The fitness of defective interfering murine coronavirus DI-a and its derivatives is described by nonsense and frameshift mutations. J Virol 1992;66:5898–5905.PubMedGoogle Scholar
  7. 7.
    Kim Y.-N., Lai M.M.C., Makino S. Generation and selection of coronavirus defective interfering RNA with large open reading frame by RNA recombination and possible editing. Virology 1993; 194:244–253.PubMedCrossRefGoogle Scholar
  8. 8.
    Leathers V, Tanguay R., Kobayashi M., Gallie D.R. A phylogenetically conserved sequence within viral 3’ untranslated RNA pseudoknots regulates translation. Mol Cell Biol 1993;13:5331–5347.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • G. D. Williams
    • 1
  • R.-Y. Chang
    • 2
  • D. A. Brian
    • 2
  1. 1.Program in BiotechnologyUniversity of TennesseeKnoxvilleUSA
  2. 2.Department of MicrobiologyUniversity of TennesseeKnoxvilleUSA

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