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Genomic Regions Associated with Neurotropism Identified in MHV by RNA-RNA Recombination

  • Ehud Lavi
  • Qian Wang
  • Susan T. Hingley
  • Susan R. Weiss
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

Infection of mice with a neurotropic strain of MHV (MHV-A59), a non-neurotropic strain of MHV (MHV-2), and a set of recombinant viruses (kindly provided by Dr. Michael Lai) were used to map genetic determinants of viral neurotropism and demyelination. Following intracerebral (IC) inoculation of 4-week old C57B1/6 mice, 1LD50 of MHV-A59 produced acute meningoencephalitis and hepatitis, and subsequently chronic CNS demyelinating disease. IC inoculation of 1LD50 of MHV-2 produced acute hepatitis without CNS disease. Recombinants ML-3, ML-11, ML-7, ML-8, ML-9 and ML-10 produced acute encephalitis similar to MHV-A59. According to previous oligonucleotide fingerprinting analysis the only common denominator of the neurotropic recombinant viruses was an M gene derived from MHV-A59. Sequencing of PCR-amplified viral S and M genes confirmed that the M genes of neurotropic viruses are derived from A59 while the S genes of neurotropic viruses are either derived from MHV-2 or from A59. In tissue culture, ML-11, ML-3 and MHV-2 are fusion negative, while A59, ML-7, ML-8 and ML-10 are fusion positive. Thus, neurotropism in MHVs is not linked to fusion or the S gene. Moreover, the M gene may be a significant determinant of neurotropism and acute encephalitis.

Keywords

Acute Hepatitis Recombinant Virus Parental Virus Mouse Hepatitis Virus Neurotropic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Ehud Lavi
    • 1
  • Qian Wang
    • 1
  • Susan T. Hingley
    • 2
  • Susan R. Weiss
    • 2
  1. 1.Division of NeuropathologyDepartment of Pathology and Laboratory MedicinePhiladelphiaUSA
  2. 2.Department of MicrobiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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