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Identification of 120 kD and 30 kD Receptors for Human Coronavirus OC43 in Cell Membrane Preparations from Newborn Mouse Brain

  • Arlene R. Collins
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

A biotinylated virus overlay was used to identify a 120kD virus-binding molecule in dissociated newborn mouse brain (nmb) cell suspensions after separation of the proteins by polyacrylamide gel electrophoresis, electroblotting, and blockage of non-specific binding sites. The virus-binding molecule was not detected in adult mouse brain cell suspensions. Mannose-and glucose-rich glycoproteins from nmb cell membranes were selected by ConA-Sepharose (Pharmacia) chromatography. A 30kD virus-binding molecule was eluted by 0.2 M alpha-methyl-D-mannoside. O-linked sialic acid, a receptor component, was identified in the eluate.

Keywords

Virus Binding Cell Membrane Preparation Solid Phase Assay Molecular Weight 120kD Cell Membrane Lysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Arlene R. Collins
    • 1
  1. 1.Department of MicrobiologyState University of New York At BuffaloBuffaloUSA

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