Identification of 120 kD and 30 kD Receptors for Human Coronavirus OC43 in Cell Membrane Preparations from Newborn Mouse Brain

  • Arlene R. Collins
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)


A biotinylated virus overlay was used to identify a 120kD virus-binding molecule in dissociated newborn mouse brain (nmb) cell suspensions after separation of the proteins by polyacrylamide gel electrophoresis, electroblotting, and blockage of non-specific binding sites. The virus-binding molecule was not detected in adult mouse brain cell suspensions. Mannose-and glucose-rich glycoproteins from nmb cell membranes were selected by ConA-Sepharose (Pharmacia) chromatography. A 30kD virus-binding molecule was eluted by 0.2 M alpha-methyl-D-mannoside. O-linked sialic acid, a receptor component, was identified in the eluate.


Virus Binding Cell Membrane Preparation Solid Phase Assay Molecular Weight 120kD Cell Membrane Lysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Pearson, J., Mims, C.A. Selective vulnerability of neural cells and age-related susceptibility to OC43 virus in mice. Archiv Virol 1983;77:109–118.CrossRefGoogle Scholar
  2. 2.
    Pearson, J., Mims, C.A. Differential susceptibility of cultured neural cells to the human coronavirus OC43. J Virol 1985;53:1016–1019.PubMedGoogle Scholar
  3. 3.
    Collins, A.R., Sorensen, O. Regulation of viral persistence in human glioblastoma and rhabdomyosarcoma cells infected with coronavirus OC43. Microb Path 1986;1:573–583.CrossRefGoogle Scholar
  4. 4.
    Collins, A.R. HLA class I antigen serves as a receptor for human coronavirus OC43. Immunol Invest 1993;22:95–103.PubMedCrossRefGoogle Scholar
  5. 5.
    Robinson, J.E., Holton, S., Liu, J., McMurdo, H., Muciano, A., Gohd, R. A novel enzyme-linked immunosorbent assay (ELISA) for the detection o antibodies to HIV-1 envelope glycoproteins based on immobilization of viral glycoproteins in microtiter wells coated with Con-A. Immunol Meth 1990;132:63–71.CrossRefGoogle Scholar
  6. 6.
    Pfleiderer, M., Routledge, E., Siddell, S.G. Functional analysis of the coronavirus MHV-JHM surface glycoproteins in vaccinia virus recombinants. Adv Virus Res 1990;276:21–31.Google Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Arlene R. Collins
    • 1
  1. 1.Department of MicrobiologyState University of New York At BuffaloBuffaloUSA

Personalised recommendations