Advertisement

Analysis of Cellular Receptors for Human Coronavirus OC43

  • C. Krempl
  • B. Schultze
  • G. Herrler
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

Bovine coronavirus (BCV), human coronavirus OC43 (HCV-OC43) and hemagglutinating encephalomyelitis virus (HEV) are serologically related viruses that all have hemagglutinating activity. The receptor determinant for attachment to erythrocytes has been shown to be N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2). We compared the ability of the three coronaviruses to recognize 9-O-acetylated sialic acid and found that they all bind to Neu5,9Ac2 attached to galactose in either A2,3 or A2,6-linkage. There are, however, some differences in the minimum amount of sialic acid that is required on the cell surface for agglutination by these viruses. Evidence is presented that HCV-OC43 uses Neu5,9Ac2 as a receptor determinant not only for agglutination of erythrocytes but also for attachment to and infection of a cultured cell line, MDCK I cells.

Keywords

Sialic Acid MDCK Cell Cellular Receptor Culture Cell Line Hemagglutinating Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Vlasak R., Luytjes W., Spaan W., Palese P. Human and bovine coronavirus recognize sialic acid containing receptors similar to those of influenza C virus. Proc Natl Acad Sci USA 1988; 85: 4526–4529.PubMedCrossRefGoogle Scholar
  2. 2.
    Schultze B., Gross H.-J., Brossmer R., Klenk H.-D. and Herrler G. Hemagglutinating encephalomyelitis virus attaches to N-acetyl-9-Oacetylneuraminic acid-containing receptors on erythrocytes: comparison with bovine coronavirus and influenza C virus. Vir.Res. 1990; 16: 185–194.CrossRefGoogle Scholar
  3. 3.
    Schultze B., Herrler G. Bovine coronavirus uses N-acetyl-9-O-acetylneuraminic acid as a receptor determinant to initiate the infection of cultured cells. J Gen Virol 1992; 73: 901–906.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • C. Krempl
    • 1
  • B. Schultze
    • 1
  • G. Herrler
    • 1
  1. 1.Institut für VirologiePhilipps-Universität MarburgMarburgGermany

Personalised recommendations