Advertisement

Analysis of the Sialic Acid-Binding Activity of Transmissible Gastroenteritis Virus

  • B. Schultze
  • L. Enjuanes
  • G. Herrler
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

Porcine transmissible gastroenteritis virus (TGEV) has been shown to agglutinate erythrocytes using a2,3-linked sialic acid on the cell surface as binding site. The hemagglutinating activity requires the pretreament of virus with neuraminidase. We obtained evidence that TGEV recognizes not only N-acetylneuraminic acid but also N-glycoloylneuraminic acid, a sialic acid present on many porcine cells.

Keywords

Sialic Acid Infectious Bronchitis Virus Hemagglutinating Activity Hemagglutination Assay Neuraminic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Spaan W., Cavanagh D. and Horzinek M.C. Coronaviruses: structure and genome expression. J.Gen.Virol. 1988; 69:2939–2952.PubMedCrossRefGoogle Scholar
  2. 2.
    Schultze B., Cavanagh D. and Herrler G. Neuraminidase treatment of avian infectious bronchitis coronavirus reveals a hemagglutinating acitivity that is dependent on sialic acid-containing receptors on erythrocytes. Virology 1992; 189: 792–794.PubMedCrossRefGoogle Scholar
  3. 3.
    Schultze B., Enjuanes L., Cavanagh D. and Herrler G. N-acetylneuraminic acid plays a critical role for the hemagglutinating activity of avian infectious bronchitis virus and porcine transmissible gastroenteritis virus. Adv.Exp.Med.Biol. 1993; 342: 305–310.PubMedCrossRefGoogle Scholar
  4. 4.
    Schultze B., Gross H.-J., Brossmer R., Klenk, H.-D. and Herrler G. Hemagglutinating encephalomyelitis virus attaches to N-acetyl-9-O-acetylneuraminic acid-containing receptors on erythrocytes: comparison with bovine coronavirus and influenza C virus. Virus Res. 1990; 16: 185–194.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • B. Schultze
    • 1
  • L. Enjuanes
    • 2
  • G. Herrler
    • 1
  1. 1.Institut für VirologiePhilipps-Universität MarburgMarburgGermany
  2. 2.Centro De Biologia MolecularCSIC Universidad AutonomaMadridSpain

Personalised recommendations