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Molecular Differentiation of Transmissible Gastroenteritis Virus and Porcine Respiratory Coronavirus Strains

Correlation with Antigenicity and Pathogenicity
  • D. J. Jackwood
  • H. M. Kwon
  • L. J. Saif
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

Transmissible gastroenteritis virus (TGEV) causes an economically important enteric disease of swine. Differences in the pathogenicity, antigenicity and tissue tropism have been observed among porcine coronaviruses. Although porcine respiratory coronavirus (PRCV) is antigenically similar but not identical to TGEV isolates, these respiratory coronaviruses differ markedly in pathogenicity and tissue tropism compared to TGEV isolates. Using a reverse transcriptase/polymerase chain reaction-restriction fragment length polymorphism (RT/PCR-RFLP) assay, TGEV and PRCV isolates were assigned to several distinct groups. By RFLP analysis of the 5’ region of the S gene, TGEV strains were differentiated into 4 groups using the restriction enzyme Sau3AI. A fifth Sau3Al group contained the PRCV isolates. These 5 groups correlated with antigenic groups previously defined using monoclonal antibodies in our laboratory. Several restriction enzymes could be used to differentiate the TGEV strains into Miller and Purdue types. Analysis of a PCR amplified product in the 3 and 3-1 genes indicated the RT/PCR-RFLP assay results for TGEV Miller strains could be correlated with lower virulence created by passage in cell culture.

Keywords

Tissue Tropism Molecular Differentiation Transmissible Gastroenteritis Virus TGEV Strain Animal Health Research 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • D. J. Jackwood
    • 1
  • H. M. Kwon
    • 1
  • L. J. Saif
    • 1
  1. 1.Food Animal Health Research Program, The Ohio Agricultural Research and Development CenterThe Ohio State UniversityWoosterUSA

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