Recombinant Expression of the TGEV Membrane Glycoprotein M

  • P. Baudoux
  • B. Charley
  • H. Laude
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)


We have previously shown that the membrane protein M of TGEV is involved in efficient induction of alpha interferon (IFNα) synthesis by non-immune peripheral blood mononuclear cells incubated with fixed, TGEV-infected cells or inactivated virions1,2. In order to determine whether M protein is able to induce interferon in the absence of other viral factors, we expressed the protein either stably in the porcine ST cells or transiently in the simian COS7 cells. Although showing no obvious difference in intracellular localization or glycosylation compared to its viral counterpart, the recombinant molecule failed to induce significant IFN activity.


COS7 Cell Recombinant Product Acute Diarrhea Proper Conformation Peripheral Blood Mononu 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • P. Baudoux
    • 1
  • B. Charley
    • 1
  • H. Laude
    • 1
  1. 1.Unite de Virologie et Immunologie MoléculairesINRAJouy-en-JosasFrance

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