Advertisement

Recombinant Expression of the TGEV Membrane Glycoprotein M

  • P. Baudoux
  • B. Charley
  • H. Laude
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

We have previously shown that the membrane protein M of TGEV is involved in efficient induction of alpha interferon (IFNα) synthesis by non-immune peripheral blood mononuclear cells incubated with fixed, TGEV-infected cells or inactivated virions1,2. In order to determine whether M protein is able to induce interferon in the absence of other viral factors, we expressed the protein either stably in the porcine ST cells or transiently in the simian COS7 cells. Although showing no obvious difference in intracellular localization or glycosylation compared to its viral counterpart, the recombinant molecule failed to induce significant IFN activity.

Keywords

COS7 Cell Recombinant Product Acute Diarrhea Proper Conformation Peripheral Blood Mononu 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Charley B., Laude H. J Virol 1988; 62:8–11.PubMedGoogle Scholar
  2. 2.
    Laude H., Gelfi J., Lavenant L., Charley B. J Virol 1992; 66:743–749.PubMedGoogle Scholar
  3. 3.
    Haelterman E.D. J Am Vet Med Assoc 1972; 160:534–540.Google Scholar
  4. 4.
    La Bonnardière C., Laude H. Infect Immun 1981; 32:28–31.PubMedGoogle Scholar
  5. 5.
    La Bonnardière C., Laude H. Ann Rech Vet 1983; 14:507–511.PubMedGoogle Scholar
  6. 6.
    Charley B., Petit E., Laude H., La Bonnardière C.Ann Virol 1983; 134E:119–126.Google Scholar
  7. 7.
    Charley B., Lavenant L., Delmas B. Scand J Immunol 1991; 33:435–440.PubMedCrossRefGoogle Scholar
  8. 8.
    Laude H., Chapsal J.M., Gelfi J., Labiau S., Grosclaude J. J Gen Virol 1986; 67:119–130.PubMedCrossRefGoogle Scholar
  9. 9.
    Laude H., Rasschaert D., Huet J.-C. J Gen Virol 1987; 68:1687–1693.PubMedCrossRefGoogle Scholar
  10. 10.
    Johansen T.E., Schøller M.S., Tolstoy S., Schwartz T.W. FEBS 1990; 267:289–294.CrossRefGoogle Scholar
  11. 11.
    Gossen M. and Bujard H. Proc Natl Acad Sci USA 1992; 89:5547–5551.PubMedCrossRefGoogle Scholar
  12. 12.
    Machamer C.E., Rose J.K.J Cell Biol 1987; 105:1205–1214.CrossRefGoogle Scholar
  13. 13.
    Machamer C.E., Mentone S.A., Rose J.K., Farquhar M.G. Proc Natl Acad Sci USA 1990; 87:6944–6948.PubMedCrossRefGoogle Scholar
  14. 14.
    Rottier P.J.M., Rose J.K. J Virol 1987; 61:2042–2045.PubMedGoogle Scholar
  15. 15.
    Armstrong J., McCrae M., Colman A. J Cell Biochem 1987; 35:129–136.PubMedCrossRefGoogle Scholar
  16. 16.
    Pulford D.J., Britton P. Virus Res 1990; 18:203–218.CrossRefGoogle Scholar
  17. 17.
    Delmas B., Laude H. Virus Res 1991; 20:107–120.PubMedCrossRefGoogle Scholar
  18. 18.
    Capobianchi M.R., Ankel H., Ameglio F., Paganelli R., Pizzoli P., Dianzani F. AIDS Res Human Retroviruses 1992; 8:575–579.CrossRefGoogle Scholar
  19. 19.
    Ito Y., Bando H., Komada H., Tsurudome M., Nishio M., Kawano H., Matsumura H., Kusagawa S., Yuasa T., Ohta H., Ikemura M., Watanabe N. J Gen Virol 1994; 75:567–572.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • P. Baudoux
    • 1
  • B. Charley
    • 1
  • H. Laude
    • 1
  1. 1.Unite de Virologie et Immunologie MoléculairesINRAJouy-en-JosasFrance

Personalised recommendations