Characterization of Structural Proteins of Lelystad Virus

  • Janneke J. M. Meulenberg
  • Annelien Petersen-den Besten
  • Eric P. de Kluyver
  • Rob J. M. Moormann
  • Wim M. M. Schaaper
  • Gert Wensvoort
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)


The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames that encode putative viral proteins. Synthetic polypeptides of 15 to 17 amino acids were selected from the amino acid sequences of ORFs 2 to 7 and anti-peptide sera were raised in rabbits. Using these anti-peptide sera and porcine anti-LV serum, we identified three structural proteins and assigned their corresponding genes. Virions were found to contain a nucleocapsid protein of 15 kDa (N), an unglycosylated membrane protein of 18 kDa (M), and a glycosylated membrane protein of 25 kDa (E). The N protein is encoded by ORF7, the M protein is encoded by ORF6, and the E protein is encoded by ORF5.


Infectious Bronchitis Virus Nucleocapsid Protein Replication Strategy CL2621 Cell Equine Arteritis Virus 
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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Janneke J. M. Meulenberg
    • 1
  • Annelien Petersen-den Besten
    • 1
  • Eric P. de Kluyver
    • 1
  • Rob J. M. Moormann
    • 1
  • Wim M. M. Schaaper
    • 2
  • Gert Wensvoort
    • 1
  1. 1.Department of VirologyInstitute for Animal Science and Health (ID-DLO)LelystadNetherland
  2. 2.Laboratory of Molecular ImmunologyInstitute for Animal Science and Health (ID-DLO)LelystadThe Netherlands

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