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Neurovirulence for Rats of the JHMV Variants Escaped from Neutralization with the S1-Specific Monoclonal Antibodies

  • Fumihiro Taguchi
  • Hideka Suzuki
  • Hiromi Takahashi
  • Hideyuki Kubo
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 380)

Abstract

We have studied the neurovirulence for rats of the MAb-resistant variants isolated from a highly neurovirulent JHMV, cl-2. The variants, MM6 and MM13, with point mutation located within the N terminal 100 amino acids (aa) of the SI protein showed no alteration in neurovirulence in comparison with cl-2, showing high neurovirulence. The variants, MM65 and MM85, with a deletion composed of about 150 aa located in the middle of the S1 subunit were revealed to be non-neurovirulent. A variant MM78 with one aa deletion, asparagic acid at number 543 from the N terminus of the S1, was shown to be low-virulence. The neurovirulence of these viruses paralleled with the viral growth potential in the rat brain. However, all of these variants as well as parental cl-2 showed high neurovirulence for mice. These results suggest that the domain composed of about 150 aa in the middle of the Sl is critical for high-neurovirulence of JHMV for rats.

Keywords

Monoclonal Antibody Point Mutation Major Determinant Growth Potential Multiple Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Fumihiro Taguchi
    • 1
  • Hideka Suzuki
    • 1
  • Hiromi Takahashi
    • 1
  • Hideyuki Kubo
    • 1
  1. 1.NCNPNational Institute of NeuroscienceKodaira TokyoJapan

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