Mapping of the RNA-Binding Domain of the Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein
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The nucleocapsid (N) protein of PRRSV is a small basic protein of 15-kDa, constituting 20‐40% of the protein content of virion (Casal et al., 1998; Snijder and Meulenberg, 1998). It is 123 amino acids in length and approximately 20% of the residues are basic (Snijder and Meulenberg, 1998). The stretches of basic amino acid regions in the PRRSV N protein are conserved and these regions have been identified as nucleolar localization signals. The related Coronavirus mouse hepatitis virus (MHV) protein interacts with RNA sequences in the leader RNA, the intergenic regions, and encapsidation signal, and this interaction is suggested to be important for transcription and encapsidation (Lai and Cavanagh, 1997).
KeywordsDeletion Mutant Nucleocapsid Protein Respiratory Syndrome Virus Mouse Hepatitis Virus Packaging Signal
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