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Functional IBV Minigenomes Generated by Recombinant Fowl Pox Viruses for use in IBV-Targeted Recombination Studies

  • Sharon Evans
  • David Cavanagh
  • Paul Britton
Chapter
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Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

Having previously demonstrated that the IBV defective (D)-RNA CD-61, can be used as an RNA vector for the expression of heterologous genes via electroporation of transcripts into infected cells (Stirrups et.al., 2000b) we wished to improve this procedure with another strategy. Hence, IBV D-RNA sequences (minigenomes) were introduced into the fowl pox virus (FPV) genome for use in a FPV/T7 system. Modified CD-61 D-RNA minigenomes were generated in situ, in a helper virus dependent manner, from cells co-infected with IBV, recombinant FPV containing CD-61 directed by the T7 promoter and rFPV expressing T7 RNA Polymerase. Efficient rescue of CD-61 D-RNAs by helper IBV was demonstrated upon serial passage in tissue culture.

Keywords

Hepatitis Delta Virus Helper Virus Hepatitis Delta Virus Ribozyme Recombination Study Chick Kidney Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Sharon Evans
    • 1
  • David Cavanagh
    • 1
  • Paul Britton
    • 1
  1. 1.Institute for Animal HealthCompton, Newbury, BerkshireEngland

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