Use of an Infectious Bronchitis Virus D-RNA as an RNA Vector
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In the absence of a complete infectious IBV cDNA we have been developing an alternative strategy for the production of recombinant IBVs, utilising a defective RNA (D-RNA), CD-61. Coronavirus D-RNAs function like a minigenome and are useful as RNA vectors for the expression of heterologous genes and for targeted recombination. IBV D-RNA CD-61 (Pénzes et al., 1996) was derived by deletion mutagenesis from a natural D-RNA, CD-91, produced by multiple passage of high titre IBV Beaudette in chick kidney (CK) cells (Pénzes et al., 1994). CD-61 lacks internal parts of the genome but contains the sequences required for replication and for packaging into virus particles and can therefore be replicated and packaged (rescued) in a helper virus-dependent manner.