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Use of an Infectious Bronchitis Virus D-RNA as an RNA Vector

  • Paul Britton
  • Kathleen Stirrups
  • Kevin Dalton
  • Kathleen Shaw
  • Sharon Evans
  • Benjamin Neuman
  • Brian Dove
  • Rosa Casais
  • Dave Cavanagh
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

In the absence of a complete infectious IBV cDNA we have been developing an alternative strategy for the production of recombinant IBVs, utilising a defective RNA (D-RNA), CD-61. Coronavirus D-RNAs function like a minigenome and are useful as RNA vectors for the expression of heterologous genes and for targeted recombination. IBV D-RNA CD-61 (Pénzes et al., 1996) was derived by deletion mutagenesis from a natural D-RNA, CD-91, produced by multiple passage of high titre IBV Beaudette in chick kidney (CK) cells (Pénzes et al., 1994). CD-61 lacks internal parts of the genome but contains the sequences required for replication and for packaging into virus particles and can therefore be replicated and packaged (rescued) in a helper virus-dependent manner.

Keywords

Infectious Bronchitis Virus Serial Passage Allantoic Fluid Chick Kidney Chick Kidney Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Paul Britton
    • 1
  • Kathleen Stirrups
    • 1
  • Kevin Dalton
    • 1
  • Kathleen Shaw
    • 1
  • Sharon Evans
    • 1
  • Benjamin Neuman
    • 1
  • Brian Dove
    • 1
  • Rosa Casais
    • 1
  • Dave Cavanagh
    • 1
  1. 1.Division of Molecular BiologyInstitute for Animal Health Compton LaboratoryCompton, NewburyUK

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