Advertisement

Mutagenesis of the 3′42 Nucleotide Host Protein Binding Element of the MHV 3′UTR

  • Reed F. Johnson
  • Julian L. Leibowitz
Chapter
  • 30 Downloads
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

The mouse hepatitis virus (MHV) genome is a 32kb message sense single stranded RNA. Studies with defective interfering RNAs have shown that the 3′ terminal 447nt are required for replication (Kim et al., 1993; Lin et al., 1993). Previous studies have identified two host protein binding elements within the 3’ terminal 166nt of the 3′UTR. The first of these is located at nucleotides 154-129 [assigning position 1 to the first nucleotide 5′ of the poly (A)] tail and the second is located within the 3′ terminal 42 nucleotides (Liu et al, 1997; Yu and Leibowitz, 1995). Both elements contain the conserved motif UGAARNGAAGUU which is required for host protein binding and for DI RNA replication (Yu and Leibowitz, 1995; Liu et al., 1997). In the present study we further identify nucleotides involved in host protein binding and explore the role of RNA secondary structure in this process.

Keywords

Secondary Structure Mouse Hepatitis Virus Secondary Structure Model Protein Binding Activity Wild Type Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Y.-N. Kim, Y. S. Jeong, and S. Makino,Analysis of cis-acting sequences essential for Coronavirus defective interfering RNA replication., Virology 197:53 (1993).PubMedCrossRefGoogle Scholar
  2. Y.-J. Lin and M. M. C. Lai, Deletion mapping of a mouse hepatitis virus defective interfering RNA reveals the requirement of an internal and discontinous sequence for replication., J.Virol. 67:6110(1993).PubMedGoogle Scholar
  3. Y. Lin, C. Liao, and M. M. C. Lai, Identification of the cis-acting signal for minus-strand RNA synthesis of a murine Coronavirus: implications for the role of minus-strand RNA in RNA replication and transcription, J.Virol. 68:8131 (1994).PubMedGoogle Scholar
  4. Q. Liu, W. Yu, and J. L. Leibowitz, A specific host cellular protein binding element near the 3′ end of mouse hepatitis virus genomic RNA., Virology 232:74 (1997).PubMedCrossRefGoogle Scholar
  5. W. Yu and J. L. Leibowitz, A conserved motif at the 3’ end of mouse hepatitis virus genomic RNA required for host protein binding and viral RNA replication., Virology 214: 128 (1995).PubMedCrossRefGoogle Scholar
  6. W. Yu and J. L. Leibowitz, Specific binding of host cellular proteins to multiple sites within the 3′ end of mouse hepatitis virus genomic RNA., J.Virol. 69:5033 (1995).Google Scholar
  7. M. Zuker, D. H. Mathews, and D. H. Turner, Algorithms and Thermodynamics for RNA Secondary Structure Prediction: A Practical Guide,. in: “RNA Biochemistry and Biotechnology”, J. Barciszewski and B. F. C. Clark, eds., Kluwer Academic Publishers, New York (1999).Google Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Reed F. Johnson
    • 1
  • Julian L. Leibowitz
    • 1
  1. 1.Department of Pathology and Laboratory MedicineThe Texas A&M University System Health Science CenterCollege StationUSA

Personalised recommendations