Mouse Hepatitis Virus Minus-strand Templates are Unstable and Turnover During Viral Replication
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During the first 6 hours after infection at 37°C, MHV-A59 infected cells accumulate seven species of minus strands, one equal in length to the viral genome RNA and six that are subgenomic and with sizes equal to those of the six subgenomic viral mRNA (Sawicki and Sawicki 1990). These templates are found in double-stranded replicative intermediates (RIs and RFs) that together with viral polymerases are active in transcription of the viral plus strand species. Thus, replication of MHV-A59 conforms to the general rules discovered for plus strand RNA viruses. It is unique in having a genome of ∼32 kb and in utilizing a novel discontinuous transcription mechanism to form the templates for its 3′ nest set of viral mRNA (Sawicki and Sawicki 1990, 1995, 1998;). Our studies have been verified by Schaad and Baric (1994) and Baric and Yount (2000).
KeywordsInfected Cell Strand Synthesis Semliki Forest Virus Viral Polymerase Minus Strand
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