Identification of the Mutations Responsible for the Phenotype of Three MHV RNA-negative ts Mutants
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A number of laboratories have isolated and characterized conditionally lethal, temperature-sensitive (ts) mutants of the A59 strain of murine hepatitis virus (MHV) (Koolen et al 1983; Sturman et al 1987; Schaad et al 1990). By and large, these studies have focused on ts mutants with an RNA-negative phenotype, i.e. mutants that are unable to synthesize viral RNA at the restrictive temperature. RNA positive mutants that are able to synthesize viral RNA but fail to produce plaques at the restrictive temperature have also been isolated and analyzed (Masters et al 1994; Ricard et al 1995; Luytjes et al 1997).