Guanosine Triphosphatase Activity of the Human Coronavirus Helicase
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RNA helicases are a diverse class of enzymes that use the energy of nucleoside triphosphate (NTP) hydrolysis to unwind duplex RNA structures. They are involved in virtually every aspect of RNA metabolism, including transcription, RNA splicing, translation, RNA export, ribosome biogenesis, mitochondrial gene expression and regulation of mRNA stability (Schmid and Linder 1992, Lohman and Bjornson 1996, Kadaré and Haenni 1997). On the basis of conserved sequence motifs, helicases have been divided into 3 large superfamilies (SF), SF1 to SF3, and two smaller families (Gorbalenya et al. 1989, Gorbalenya and Koonin 1993b), which contain both RNA and DNA helicases.
KeywordsBaculovirus Recombinant Helicase Domain Conserve Sequence Motif Magnesium Acetate Recombinant Baculoviruses
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