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Are Intestinal Mucins Involved in the Pathogenicity of Transmissible Gastroenteritis Coronavirus?

  • Christel Scwegmann
  • Gert Zimmer
  • Georg Herrler
Chapter
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Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

Transmissible gastroenteritis virus (TGEV) is a prototype of enteropathogenic coronaviruses. The virus causes diarrhea in pigs of all ages. Infections are most severe in newborn piglets where letality can be as high as 100% (Pensaert et al 1993). The determinants of the enterotropism of TGEV are not known. A crucial factor appears to be the sialic acid binding activity of this virus (Schultze et al 1996). The ability of TGEV to attach to sialoglycoconjugates allows the virus to bind to erythrocytes. This interaction results in an agglutination reaction that probably has no physiological importance. However, hemagglutination provides a convenient assay for the sialic acid binding activity and allows quantitation of the virus. Mutants of TGEV that have lost their haemagglutinating activity because of a single point mutation in the S protein also had lost enteropathogenicity (Krempl et al 1997). Porcine respiratory Coronavirus (PRCV), a respiratory variant of TGEV also lacks hemagglutinating activity. Both PRCV and the hemagglutination-deficient mutants are still able to grow in cell culture. Therefore, the sialic acid binding activity appears to be essential for enteropathogenicity but dispensible for growth in cultured cells. The sialic acid binding activity is located on the viral surface protein S. Another binding activity of this glycoprotein is the ability to interact with aminopeptidase N, the cellular receptor for TGEV (Delmas et al 1992). PRCV and the hemagglutination-deficient mutants have retained the ability to bind to aminopeptidase N. Therefore, the interaction with aminopeptidase N — though essential for the infection of cells — does not explain the enteropathogenicity of TGEV.

Keywords

High Performance Liquid Chromatography Sialic Acid Newborn Piglet Sialic Acid Content Transmissible Gastroenteritis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Delmas, B., Gelfi, J., L’Haridon, R., Vogel, L. K., Sjöström, H., Noren, O. and Laude, H., 1992, Aminopeptidase N is a major receptor for the entero-pathogenic Coronavirus TGEV. Nature 357:411–420.CrossRefGoogle Scholar
  2. Enss, M.-L., Schmidt-Wittig, U., Müller, H., Mai, U. E. H., Coenen, M. and Hedrich, H. J., 1996, Response of germfree rat colonie mucous cells to peroral endotoxin application. European Journal of Cell Biology 71:99–104.PubMedGoogle Scholar
  3. Krempl, C., Schultze, B., Laude, H. and Herrler, G., 1997, Point mutations in the S protein connect the sialic acid binding activity with the enteropathogenicity of transmissible gastroenteritis Coronavirus. Journal of Virology 71:3285–3287.PubMedGoogle Scholar
  4. Krempl, C., Ballesteros, M. L., Zimmer, G., Enjuanes, L., Klenk, H.-D. and Herrler, G., 2000, Characterization of the sialic acid binding activity of transmissible gastroenteritis Coronavirus by analysis of haemagglutination-deficient mutants. Jounal of General Virology 81:489–496.Google Scholar
  5. Pensaert, M., Callebaut, P. and Cox, E., 1993, Enteric coronaviruses of animals. In Viral Infections of the Gastrointestinal Tract. Edited by A. Z. Kapikian. New York, Marcel Dekker, pp. 627–696.Google Scholar
  6. Schultze, B. and Herrler, G., 1992, Bovine Coronavirus uses N-acetyl-9-O-acetylneuraminic acid as a receptor determinant to initiate the infection of cultured cells. Journal of General Virology 73:901–906.PubMedCrossRefGoogle Scholar
  7. Schultze, B., Krempl, C., Ballesteros, M. L., Shaw, L., Schauer, R., Enjuanes, L. and Herrler, G., 1996, Transmissible gastroenteritis Coronavirus, but not the related porcine respiratory Coronavirus, has a sialic acid (N-glycolylneuraminic acid) binding activity. Journal of Virology 70:5634–5637.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Christel Scwegmann
    • 1
  • Gert Zimmer
    • 1
  • Georg Herrler
    • 1
  1. 1.Institut für VirologieTierärztliche Hochschule HannoverHannoverGermany

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