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Demyelination Determinants in the S Gene of MHV

  • Jayasri Das Sarma
  • Li Fu
  • Susan R. Weiss
  • Ehud Lavi
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 494)

Abstract

Demyelination is the hallmark pathologic process of several viral-induced, and inflammatory, immune-mediated diseases of the central nervous system (Allen and Bradkin 1993). Several experimental animal model systems are used to study demyelination, including MHV-induced demyelination in mice (Stohlman et al 1981; Wege et al 1982; Lavi et al 1984; Lavi et al 1984b; Lavi and Weiss 1989; Houtman et al 1996; Lavi et al 1999). Previous studies with this model suggested that the S protein and gene may be important for pathogenesis since the spike (S) protein participates in many functions related to virus-host interactions (Dalziel et al 1986). Thus we wanted to explore the possibility that the S gene contains molecular determinants of demyelination. We used targeted RNA recombination as previously described (Peng et al 1995; Leparc-Goffart et al 1998; Phillips et al 1999) to insert the S gene of a non-demyelinating virus (MHV-2) into the background of a demyelinating virus (MHV-A59). We then studied the pathogenesis of the new recombinant viruses.

Keywords

Viral Antigen Recombinant Virus Viral Persistence Murine Hepatitis Virus Murine Coronavirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Jayasri Das Sarma
    • 1
  • Li Fu
    • 1
  • Susan R. Weiss
    • 2
  • Ehud Lavi
    • 1
  1. 1.Department of Pathology and Laboratory MedicineUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.Department of MicrobiologyUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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