Apoptosis in the Lungs of Pigs During an Infection with a European Strain of Porcine Reproductive and Respiratory Syndrome Virus
- 31 Downloads
It has been shown, in vitro, that porcine reproductive and respiratory syndrome virus (PRRSV) induces apoptosis during its replication in both MA-104 cells and porcine alveolar macrophages (Suarez et al., 1996). Since transfection of mammalian cells with ORF5 of PRRSV leads to apoptosis it was suggested that the 25-kDa glycosylated membrane protein GP5 of PRRSV is responsible for this phenomenon. Apoptosis has also been demonstrated in vivo with North-American strains of PRRSV (Sur et al, 1997, 1998; Sirinarumitr et al., 1998). Apoptotic cells were localized in germinal epithelial cells of testicles (Sur et al, 1997), in lungs and in lymphoid tissues of pigs (Sirinarumitr et al., 1998; Sur et al., 1998). The apoptotic cells were morphologically recognized as alveolar and pulmonary intravascular macrophages and mononuclear cells in the alveolar septa in lungs and as macrophages and mononuclear cells in lymph nodes. Apoptotic cells were more abundant than PRRSV-infected cells in all tissues and double-labeling experiments indicated that the majority of apoptotic cells were uninfected bystander cells.
KeywordsPost Inoculation Cryostat Section Chicken Anaemia Virus Lelystad Virus Porcine Alveolar Macrophage
- Wensvoort, G., Terpstra, C., Pol, J. M. A., ter Laak, E. A., Bloemraad, M., de Kluyver, E. P., Kragten, C., van Buiten, L., den Besten, A., Wagenaar, F., Broekhuijsen, J. M., Moonen, P.LJ. M., Zetstra, T., de Boer, E. A., Tibben, H. J., de Jong, M. F., van’ t Veld, P., Groenland, G. J. R., van Gennep, J. A., Voets, M. T., Verheijden, J. H. M., and Braamskamp, J., 1991, Vet.Q., 13: 121–130.PubMedCrossRefGoogle Scholar