Alteration of the Inflammatory Molecule Network After Irradiation of Soft Tissue

  • Zhenyu Xiao
  • Shanmin Yang
  • Ying Su
  • Wei Wang
  • Hengshan Zhang
  • Mei Zhang
  • Kunzhong Zhang
  • Yeping Tian
  • Yongbing Cao
  • Liangjie Yin
  • Lurong Zhang
  • Paul Okunieff
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 765)

Abstract

Inflammatory molecules (IMs) play an important role in ionizing radiation (IR)-induced soft tissue damage. The alteration of IMs as a function of time was studied with a protein array containing 62 IMs in mouse cutaneous soft tissues exposed to 30 Gy. The results showed that: (1) 2 days after irradiation, the levels of TGF-β1, MIP-1γ, IL-1α, and sTNF RI increased, while IGFBP-3, CXCL16, and IL-1β decreased in IR skin as compared to control skin; (2) 21 days after IR, TGF-β1, and MIP-1 γ, IL-1α remained high, while CXCL16 and IL-1β remained low; (3) 3 months after IR, the cytokine pattern exhibited reversals. The levels of MIP-1γ decreased, while VCAM-1, IGFBP-3, and TGF-β1 production increased. The data indicated that: (a) IMs change as a function of time after soft tissue irradiation; (b) changing IM levels may reflect the altered balance of the cytokine network, leading to imbalance or homeostasis; and (c) an antibody-based protein array can be used to assess multiple IMs simultaneously, making it useful for bulk screening for changes in tissue cytokine levels.

Keywords

Inflammatory molecules Protein array Soft tissue Cytokines Radiation 

Notes

Acknowledgments

This project is supported in part by U19 AI067733. We thank Kate Casey-Sawicki for editing this manuscript.

References

  1. 1.
    Rubin P, Johnston CJ, Williams JP et al (1995) A perpetual cascade of cytokines postirradiation leads to pulmonary fibrosis. Int J Radiat Oncol Biol Phys 33:99–109CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Burmeister BH, Smithers BM, Davis S et al (2002) Radiation therapy following nodal surgery for melanoma: an analysis of late toxicity. ANZ J Surg 72:344–348CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Okunieff P, Augustine E, Hicks JE et al (2004) Pentoxifylline in the treatment of radiation-induced fibrosis. J Clin Oncol 22:2207–2213CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Grimberg A, Coleman CM, Burns TF et al (2005) p53-Dependent and p53-independent induction of insulin-like growth factor binding protein-3 by deoxyribonucleic acid damage and hypoxia. J Clin Endocrinol Metab 90:3568–3574CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Molla M, Gironella M, Miquel R et al (2003) Relative roles of ICAM-1 and VCAM-1 in the pathogenesis of experimental radiation-induced intestinal inflammation. Int J Radiat Oncol Biol Phys 57:264–273CrossRefGoogle Scholar
  6. 6.
    Epperly MW, Sikora CA, DeFilippi SJ et al (2002) Pulmonary irradiation-induced expression of VCAM-I and ICAM-I is decreased by manganese superoxide dismutase-plasmid/liposome (MnSOD-PL) gene therapy. Biol Blood Marrow Transplant 8:175–187CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Rajalalitha P, Vali S (2005) Molecular pathogenesis of oral submucous fibrosis—a collagen metabolic disorder. J Oral Pathol Med 34:321–328CrossRefPubMedGoogle Scholar
  8. 8.
    Martin M, Lefaix J, Delanian S (2000) TGF-beta1 and radiation fibrosis: a master switch and a specific therapeutic target? Int J Radiat Oncol Biol Phys 47:277–290CrossRefPubMedGoogle Scholar
  9. 9.
    Baxter RC (2001) Signalling pathways involved in antiproliferative effects of IGFBP-3: a review. Mol Pathol 54:145–148CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Zhenyu Xiao
    • 1
  • Shanmin Yang
    • 1
  • Ying Su
    • 1
  • Wei Wang
    • 1
  • Hengshan Zhang
    • 1
  • Mei Zhang
    • 1
  • Kunzhong Zhang
    • 1
  • Yeping Tian
    • 1
  • Yongbing Cao
    • 1
  • Liangjie Yin
    • 1
  • Lurong Zhang
    • 1
  • Paul Okunieff
    • 1
  1. 1.Department of Radiation Oncology, UF Shands Cancer CenterUniversity of FloridaGainesvilleUSA

Personalised recommendations