Preservation of Hemopoietic Stem Cells
Hemopoiesis in mammals is maintained by continuing proliferation and differentiation of putative pluripotential hemopoietic stem cells (PHSC). Such cells cannot be recognized by morphological or immunological techniques but can be defined operationally in experimental systems and clinical studies. Their presence in normal bone marrow (and probably also in the peripheral blood), from which site they can be harvested by appropriate techniques, has formed the basis for treating patients with various types of hematological or neoplastic disease by transplantation of allogeneic or autologous bone marrow cells. In this paper I will attempt to define the PHSC, to describe some of the methods by which such cells may be collected for clinical use, to refer to methods for storing stem cells and outline the results of their use in clinical practice.
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