The Use of Multiply Marked Escherichia coli K12 Strains in the Host-Mediated Assay

  • G. R. Mohn
  • P. R. M. Kerklaan
  • P. A. van Elburg


Due to the development in the last decade of rapid and sensitive bacterial genetic test systems for detecting potential mutagens and carcinogens(1–3) there has been a recrudescence of testing of environmental chemicals for genotoxicity. Experiments performed in particular with theSalmonella/mammalian microsome test,(4,5) in which the ability of chemicals to cause reversion in auxotrophicSalmonella strains is determined on selective agar medium under the influence of various mammalian organ fractions, have yielded results on a variety of environmental compounds that show definite mutagenic activity in this assay system and are therefore to be considered as potentially mutagenic and carcinogenic in animals (see also Refs. 6 and 7).


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© Springer Science+Business Media New York 1984

Authors and Affiliations

  • G. R. Mohn
    • 1
  • P. R. M. Kerklaan
    • 1
  • P. A. van Elburg
    • 1
  1. 1.Department of Radiation Genetics and Chemical MutagenesisState University of LeidenAL LeidenThe Netherlands

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