The Bacillus subtilis Multigene Sporulation Test for Detection of Environmental Mutagens

  • L. E. Sacks
  • J. T. MacGregor


The value of short-term in vitro screening tests for environmental mutagens is well established. It is generally agreed that a small battery of tests using both prokaryotic and eukaryotic cells should be used in preliminary screening to detect potential mutagens.(7) Bacterial tests are efficient and economical methods of detecting specific locus mutations. Moreover, well-developed methods of genetic manipulation make it easier to study mechanisms in bacteria. Tests for general genetic screening should be sensitive to a wide range of genetic damage and, if possible, representative of damage to the genome as a whole.


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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • L. E. Sacks
    • 1
  • J. T. MacGregor
    • 1
  1. 1.United States Department of AgricultureWestern Regional Research CenterBerkeleyUSA

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