In Vivo and in Vitro Models of Demyelinating Disease

Factors Influencing the Disease Process Caused by Coronavirus Infection of Rats
  • O. Sorensen
  • S. Beushausen
  • M. Coulter-Mackie
  • R. Adler
  • S. Dales


Murine coronaviruses, including MHV-A59, MHV-3, and JHM, have a neurotropic potential in rodents causing either an acute fatal encephalitis or a paralytic disease (Bailey et al., 1949; Lampert et al., 1973; Le Provost et al., 1975; Hirano et al., 1980; Knobler et al, 1981b). Central nervous system (CNS) infection of mice may result from intracerebral (IC) or intraperitoneal (IP) inoculation of a number of these coronaviruses, which have tropisms for various CNS cells including glia and neurons (Virelizier et al., 1975; Fleury et al., 1980; Knobler et al., 1981a). By contrast, in rats overt CNS symptoms are associated exclusively with JHM virus (JHMV) (Hirano et al., 1980; Sorensen et al., 1980). An IC inoculation of MHV-3 into rats may result in virus replication without overt symptoms of disease (Hirano et al., 1980; our unpublished data), implying that this strain replicates in cells that are not critical for CNS function, at least as judged by clinical criteria. Although MHV-3 infection of the rat CNS appears to be transitory, JHMV RNA can be identified in the CNS of some asymptomatic animals several months post-inoculation (Sorensen et al., 1984). It remains to be established whether, under these circumstances, JHMV persists at low titers as an infectious entity or enters into a truly latent state.


Demyelinating Disease Central Nervous System Tissue Central Nervous System Cell Mouse Hepatitis Virus Wistar Furth 
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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • O. Sorensen
    • 1
  • S. Beushausen
    • 1
  • M. Coulter-Mackie
    • 1
  • R. Adler
    • 1
  • S. Dales
    • 1
  1. 1.Cytobiology Group, Department of Microbiology and ImmunologyUniversity of Western OntarioLondonCanada

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