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In Vivo and in Vitro Models of Demyelinating Disease

Factors Influencing the Disease Process Caused by Coronavirus Infection of Rats
  • O. Sorensen
  • S. Beushausen
  • M. Coulter-Mackie
  • R. Adler
  • S. Dales
Chapter

Abstract

Murine coronaviruses, including MHV-A59, MHV-3, and JHM, have a neurotropic potential in rodents causing either an acute fatal encephalitis or a paralytic disease (Bailey et al., 1949; Lampert et al., 1973; Le Provost et al., 1975; Hirano et al., 1980; Knobler et al, 1981b). Central nervous system (CNS) infection of mice may result from intracerebral (IC) or intraperitoneal (IP) inoculation of a number of these coronaviruses, which have tropisms for various CNS cells including glia and neurons (Virelizier et al., 1975; Fleury et al., 1980; Knobler et al., 1981a). By contrast, in rats overt CNS symptoms are associated exclusively with JHM virus (JHMV) (Hirano et al., 1980; Sorensen et al., 1980). An IC inoculation of MHV-3 into rats may result in virus replication without overt symptoms of disease (Hirano et al., 1980; our unpublished data), implying that this strain replicates in cells that are not critical for CNS function, at least as judged by clinical criteria. Although MHV-3 infection of the rat CNS appears to be transitory, JHMV RNA can be identified in the CNS of some asymptomatic animals several months post-inoculation (Sorensen et al., 1984). It remains to be established whether, under these circumstances, JHMV persists at low titers as an infectious entity or enters into a truly latent state.

Keywords

Demyelinating Disease Central Nervous System Tissue Central Nervous System Cell Mouse Hepatitis Virus Wistar Furth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • O. Sorensen
    • 1
  • S. Beushausen
    • 1
  • M. Coulter-Mackie
    • 1
  • R. Adler
    • 1
  • S. Dales
    • 1
  1. 1.Cytobiology Group, Department of Microbiology and ImmunologyUniversity of Western OntarioLondonCanada

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