Overproduction of Nitric Oxide in Physiology and Pathophysiology: EPR Detection

  • Yann A. Henry


Stimulating developments in current research on the biological roles of NO are the immediate implications to physiology and pathophysiology, both in animal models and in humans.1–3 Many diseases have been related to overproduction or underproduction of NO by NOS isoenzymes. The use of NO-donors, such as organic nitrates, nitroprusside or molsidomines, has in fact largely preceded the discovery of EDRF/NO and is quite well monitored (see chapter 12). The use of NO gas and that of NOS inhibitors have been under careful trial for several diseases since 1990. We shall limit the evocation of NO-related pathophysiology in animal models and in humans to a miscellaneous and restricted number of cases in which EPR spectroscopy has made some contribution to the estimation or quantification of NO: cancer, infections and septic shock, autoimmune diseases, organ transplantations and a few other cases in which NOS II induction occurs. Whenever possible we shall focus on human pathologies. Due to the low sensitivity of EPR spectroscopy, most of the cases of detection derive from NO synthesized by inducible NOS in a long-term process which allows NO accumulation on metalloprotein targets. Several selective reviews have been published on this subject.4–9


Nitric Oxide Cerebral Malaria Experimental Allergic Encephalomyelitis Nitric Oxide Synthesis Endotoxic Shock 
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© R.G. Landes Company 1997

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  • Yann A. Henry

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