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Characterization of a New Mobilizable Mac-1 (CD11b/CD18) Pool That Co-Localizes with Gelatinase in Human Neutrophils

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Leukocyte Adhesion Molecules

Abstract

The CD11/CD18 complex consists of three glycoprotein heterodimers [Mac-1, LFA-1, and p150,95 (1–3)] that share identical β subunits non-covalently associated with immunologically distinct α subunits. Mac-1 (CD11b/CD18) is the receptor for iC3b opsonized particles (4–6) and plays a major role in adhesion and adhesion-dependent functions of human myeloid cells (4,7–9). In normal unstimulated neutrophils and monocytes, Mac-1 is present in intracellular compartments as well as on the cell surface (3,10–13). Exposure to chemotactic stimuli (C5a, f-Met-Leu-Phe [fMLP], or Leukotriene B4) or secretagogues (phorbol myristate acetate [PMA] or calcium ionophore A23187) elicits a 5- to 10-fold increase in surface binding of monoclonal antibodies (MAbs) directed at Mac-1 (3,4,14,15) associated with a two to threefold increase in neutrophil adhesiveness (7,16). This surface protein “upregulation” is maximal within 10 minutes at 37 °C and is not impeded by protein or mRNA synthesis inhibitors, suggesting that Mac-1 is translocated from latent intracellular pools to the cell surface in response to inflammatory stimuli.

Supported by National Institutes of Health Grants No. AI19031, AI23521, DE07875, and HL41408, the National Cystic Fibrosis Foundation, and by the US Department of Agriculture-Agricultural Research Service/Children’s Nutrition and Research Center, Baylor College of Medicine and Texas Children’s Hospital. Dr. Anderson was the recipient of a Research Career Development Award (K04-AI/AM 00105) during the performance of these investigations.

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Authors
  1. Douglas H. Jones
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  2. Frank C. Schmalstieg
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  3. Hal K. Hawkins
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  4. Bean L. Burr
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  5. Helen E. Rudloff
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  6. Sharon Krater
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  7. C. Wayne Smith
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  8. Donald C. Anderson
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Editor information

Editors and Affiliations

  1. Center for Blood Research, Harvard Medical School, 800 Huntington Avenue, Boston, Massachusetts, 02115, USA

    Timothy A. Springer Ph.D. (Associate Professor) (Associate Professor)

  2. Pediatrics, Microbiology and Immunology, Head, Leukocyte Biology Section, Baylor College of Medicine and Texas Children’s Hospital, 8080 North Stadium, Suite #2100, Houston, Texas, 77054, USA

    Donald C. Anderson M.D. (Professor) (Professor)

  3. Department of Immunology, Boehringer Ingelheim Pharmaceuticals, Inc., 90 East Ridge Road, Box 368, Ridgefield, Connecticut, 06877, USA

    Robert Rothlein Ph.D. (Senior Principal Scientist) (Senior Principal Scientist)

  4. Research & Development, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, Connecticut, 06877, USA

    Alan S. Rosenthal (Vice President) (Vice President)

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© 1990 Springer-Verlag New York Inc.

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Jones, D.H. et al. (1990). Characterization of a New Mobilizable Mac-1 (CD11b/CD18) Pool That Co-Localizes with Gelatinase in Human Neutrophils. In: Springer, T.A., Anderson, D.C., Rothlein, R., Rosenthal, A.S. (eds) Leukocyte Adhesion Molecules. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3234-6_9

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  • DOI: https://doi.org/10.1007/978-1-4612-3234-6_9

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7927-3

  • Online ISBN: 978-1-4612-3234-6

  • eBook Packages: Springer Book Archive

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