A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification
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In principle, PCR generates large quantities of DNA from a minute amount of nucleic acid starting material using a methodology similar to (but much simpler than) that seen in living cells. For living cells, in vivo DNA synthesis is dependent upon a well defined but complex set of enzymes and co-factors, which have evolved to act in a concerted fashion during the synthetic phase (S-phase) of the cell cycle. In comparison, PCR facilitates in vitro DNA synthesis in a much simpler fashion, making use of a smaller set of defined ingredients and reaction conditions involving relatively high temperatures. The range of factors contributing to successful PCR amplification is reviewed below.
KeywordsExonuclease Activity Deoxynucleotide Triphosphate Exonuclease Domain Concerted Fashion Single Strand Binding Protein
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