Characterization of Persistent SARS-CoV Infection in Vero E6 Cells

  • Tetsuya Mizutani
  • Shuetsu Fukushi
  • Masayuki Saijo
  • Ichiro Kurane
  • Shigeru Morikawa
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 581)


Severe Acute Respiratory Syndrome Persistent Infection Severe Acute Respiratory Syndrome Sindbis Virus Infected Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Rota, P. A., Oberste, M. S., Monroe, S. S., et al., 2003, Characterization of a novel coronavirus associated with severe acute respiratory syndrome, Science 300:1394–1399.CrossRefPubMedGoogle Scholar
  2. 2.
    Marra, M. A., Jones, S. J., Astell, C. R., et al., 2003, The genome sequence of the SARS-associated coronavirus, Science 300:1399–1404.CrossRefPubMedGoogle Scholar
  3. 3.
    Chan P. K., To, K. F., Lo, A. W., Cheung, J. L., Chu, I., Au, F. W., Tong, J. H., Tam, J. S., Sung, J. J., and Ng, H. K., 2004, Persistent infection of SARS coronavirus in colonic cells in vitro, J. Med. Virol. 74:1–7.CrossRefPubMedGoogle Scholar
  4. 4.
    Li, W., Moore, M. J., Vasilieva, N., et al., 2003, Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus, Nature 426:450–454.CrossRefPubMedGoogle Scholar
  5. 5.
    Mizutani, T., Fukushi, S., Saijo, M., Kurane, I., and Morikawa, S., 2004a, Phosphorylation of p38 MAPK and its downstream targets in SARS coronavirus-infected cells, Biochem. Biophys. Res. Commun. 319:1228–1234.CrossRefPubMedGoogle Scholar
  6. 6.
    Mizutani, T., Fukushi, S., Saijo, M., Kurane, I., and Morikawa, S., 2004b, Importance of Akt signaling pathway for apoptosis in SARS-CoV-infected Vero E6 cells, Virology 327:169–174.CrossRefPubMedGoogle Scholar
  7. 7.
    Mizutani, T., Fukushi, S., Murakami, M., Hirano, T., Saijo, M., Kurane, I., and Morikawa, S., 2004c. Tyrosine dephosphorylation of STAT3 in SARS coronavirus-infected Vero E6 cells, FEBS Lett. 577:187–192.CrossRefPubMedGoogle Scholar
  8. 8.
    Mizutani, T., Fukushi, S., Saijo, M., Kurane, I., and Morikawa, S., 2005, JNK and PI3K/Akt signaling pathways are required for establishing persistent SARS-CoV-infection in Vero E6 cells, Biochim. Biophys. Acta. 1741: 4–10.PubMedGoogle Scholar
  9. 9.
    Liao C. L., Lin, Y. L., Shen, S. C., Shen, J. Y., Su, H. L., Huang, Y. L., Ma, S. H., Sun, Y. C., Chen, K. P. and Chen, L. K. 1998, Antiapoptic but not antiviral function of human bcl-2 assists establishment of Japanese encephalitis virus persistence in cultured cells. J Virol. 72:9844–9854.PubMedGoogle Scholar
  10. 10.
    Appel, E., Katzoff, A., Ben-Moshe, T., Kazimirsky, G., Kobiler, D., Lustig, S., and Brodie, C., 2000, Differential regulation of Bcl-2 and Bax expression in cells infected with virulent and nonvirulent strains of sindbis virus, Virology 276:238–242.CrossRefPubMedGoogle Scholar
  11. 11.
    Poruchynsky, M. S., Wang, E. E., Rudin, C. M., Blagosklonny, M. V., and Fozo, T., 1998, Bcl-xL is phosphorylated in malignant cells following microtubule disruption, Cancer Res. 58:3331–3338.PubMedGoogle Scholar
  12. 12.
    Kharbanda, S., Saxena, S., Yoshida, K., et al., 2000, Translocation of SAPK/JNK to mitochondria and interaction with Bcl-x(L) in response to DNA damage, J. Biol. Chem. 275:322–327.CrossRefPubMedGoogle Scholar
  13. 13.
    Basu, A., and Haldar, S., 2003, Identification of a novel Bcl-xL phosphorylation site regulating the sensitivity of taxol- or 2-methoxyestradiol-induced apoptosis, FEBS Lett. 538:41–47.CrossRefPubMedGoogle Scholar
  14. 14.
    Hinshaw, V. S., Olsen, C. W., Dybdahl-Sissoko, N., and Evans, D., 1994, Apoptosis: a mechanism of cell killing by influenza A and B viruses, J. Virol. 68:3667–3673.PubMedGoogle Scholar
  15. 15.
    Ubol, S., Tucker, P. C., Griffin, D. E, and Hardwick, J. M., 1994, Neurovirulent strains of alphavirus induce apoptosis in bcl-2-expressing cells: role of a single amino acid change in the E2 glycoprotein, Proc. Natl. Acad. Sci. USA 91:5202–5206.CrossRefPubMedGoogle Scholar
  16. 16.
    Pekosz, A., Phillips, J., Pleasure, D., Merry, D., and Gonzalez-Scarano, F., 1996, Induction of apoptosis by La Crosse virus infection and role of neuronal differentiation and human bcl-2 expression in its prevention, J. Virol. 70:5329–5335.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Tetsuya Mizutani
    • 1
  • Shuetsu Fukushi
    • 2
  • Masayuki Saijo
    • 3
  • Ichiro Kurane
    • 4
  • Shigeru Morikawa
    • 5
  1. 1.National Institute of Infectious DiseasesJapan
  2. 2.National Institute of Infectious DiseasesJapan
  3. 3.National Institute of Infectious DiseasesJapan
  4. 4.National Institute of Infectious DiseasesJapan
  5. 5.National Institute of Infectious DiseasesJapan

Personalised recommendations