Regulation of Coronavirus Transcription: Viral and Cellular Proteins Interacting with Transcription-Regulating Sequences

  • Sonia Zúñiga
  • Isabel Sola
  • Jose L. Moreno
  • Sara Alonso
  • Luis Enjuanes
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 581)


Electrophoretic Mobility Shift Assay Cleavage Reaction Nucleocapsid Protein Hepatitis Delta Virus Template Switch 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    S. G. Sawicki and D. L. Sawicki, A new model for coronavirus transcription, Adv. Exp. Med. Biol. 440, 215–220 (1998).PubMedGoogle Scholar
  2. 2.
    I. Sola, J. L. Moreno, S. Zúñiga, S. Alonso, and L. Enjuanes, Role of nucleotides immediately flanking the transcription-regulating sequence core in coronavirus subgenomic mRNA synthesis, J. Virol. 79, 2506–2516 (2005).CrossRefPubMedGoogle Scholar
  3. 3.
    S. Zúñiga, I. Sola, S. Alonso, and L. Enjuanes, Sequence motifs involved in the regulation of discontinuous coronavirus subgenomic RNA synthesis, J. Virol. 78, 980–994 (2004).CrossRefPubMedGoogle Scholar
  4. 4.
    F. Almazán, C. Galán, and L. Enjuanes, The nucleoprotein is required for efficient coronavirus genome replication, J. Virol. 78, 12683–12688 (2004).CrossRefPubMedGoogle Scholar
  5. 5.
    G. Cristofari and J. L. Darlix, The ubiquitous nature of RNA chaperone proteins, Prog. Nucleic Acid Res. Mol. Biol. 72, 223–268 (2002).CrossRefPubMedGoogle Scholar
  6. 6.
    D. Herschlag, RNA chaperones and the RNA folding problem, J. Biol. Chem. 270, 20871–2084 (1995).PubMedGoogle Scholar
  7. 7.
    J. R. Lorsch, RNA chaperones exist and DEAD box proteins get a life, Cell 109, 797–800 (2002).CrossRefPubMedGoogle Scholar
  8. 8.
    R. Schroeder, A. Barta, and K. Semrad, Strategies for RNA folding and assembly, Nat. Rev. Mol. Cell. Biol. 5, 908–919 (2004).CrossRefPubMedGoogle Scholar
  9. 9.
    E. L. Bertrand and J. J. Rossi, Facilitation of hammerhead ribozyme catalysis by the nucleocapsid protein of HIV-1 and the heterogeneous nuclear ribonucleoprotein A1, EMBO. J. 13, 2904–2912 (1994).PubMedGoogle Scholar
  10. 10.
    Z. Tsuchihashi and P. O. Brown, DNA strand exchange and selective DNA annealing promoted by the Human Immunodeficiency Virus Type I nucleocapsid protein, J. Virol. 68, 5863–5870 (1994).PubMedGoogle Scholar
  11. 11.
    Z. S. Huang and H. N. Wu, Identification and characterization of the RNA chaperone activity of hepatitis delta antigen peptides, J. Biol. Chem. 273, 26455–26461 (1998).CrossRefPubMedGoogle Scholar
  12. 12.
    Z. S. Huang, W. H. Su, J. L. Wang, and H. N. Wu, Selective strand annealing and selective strand exchange promoted by the N-terminal domain of hepatitis delta antigen, J. Biol. Chem. 278, 5685–5693 (2003).CrossRefPubMedGoogle Scholar
  13. 13.
    G. Cristofari, R. Ivanyi-Nagy, C. Gabus, et al., The hepatitis C virus Core protein is a potent nucleic acid chaperone that directs dimerization of the viral (+) strand RNA in vitro, Nucleic Acids Res. 32, 2623–2631 (2004).CrossRefPubMedGoogle Scholar
  14. 14.
    R. Ivanyi-Nagy, L. Davidovic, E. W. Khandjian, and J. L. Darlix, Disordered RNA chaperone proteins: from functions to disease, Cell. Mol. Life Sci. 62, 1409–1417 (2005).CrossRefPubMedGoogle Scholar
  15. 15.
    J. Xie, J. Lee, T. L. Kress, K. L. Mowry, and D. L. Black, Protein kinase A phosphorylation modulates transport of the polypyrimidine tract-binding protein, Proc. Natl. Acad. Sci. USA 100, 8776–8781 (2003).CrossRefPubMedGoogle Scholar
  16. 16.
    J. A. Daròs and R. Flores, A chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage, EMBO. J. 21, 749–759 (2002).CrossRefPubMedGoogle Scholar
  17. 17.
    J. M. Martín-Alonso, M. Balbín, D. J. Garwes, L. Enjuanes, S. Gascón, and F. Parra, Antigenic structure of transmissible gastroenteritis virus nucleoprotein, Virology 188, 168–174 (1992).CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Sonia Zúñiga
    • 1
  • Isabel Sola
    • 2
  • Jose L. Moreno
    • 3
  • Sara Alonso
    • 4
  • Luis Enjuanes
  1. 1.Centro Nacional de BiotecnologíaCSICSpain
  2. 2.Centro Nacional de BiotecnologíaCSICSpain
  3. 3.Centro Nacional de BiotecnologíaCSICSpain
  4. 4.Centro Nacional de BiotecnologíaCSICSpain

Personalised recommendations