Attachment Factor and Receptor Engagement of Sars Coronavirus and Human Coronavirus NL63

  • Heike Hofmann
  • Andrea Marzi
  • Thomas Gramberg
  • Martina Geier
  • Krzysztof Pyrc
  • Lia van der Hoek
  • Ben Berkhout
  • Stefan Pöhlmann
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 581)


Membrane Fusion Heptad Repeat Neutralize Antibody Response Cell Tropism Cellular Entry 
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  1. 1.
    H. Hofmann and S. Pöhlmann, Cellular entry of the SARS coronavirus, Trends Microbiol. 12, 466–472 (2004).CrossRefPubMedGoogle Scholar
  2. 2.
    A. E. Smith and A. Helenius, How viruses enter animal cells, Science 304, 237–242 (2004).CrossRefPubMedGoogle Scholar
  3. 3.
    D. M. Eckert and P. S. Kim, Mechanisms of viral membrane fusion and its inhibition, Annu. Rev. Biochem. 70, 777–810 (2001).CrossRefPubMedGoogle Scholar
  4. 4.
    F. X. Heinz, K. Stiasny, and S. L. Allison, The entry machinery of flaviviruses, Arch. Virol. Suppl. (18), 133-137 (2004).Google Scholar
  5. 5.
    D. J. Schibli and W. Weissenhorn, Class I and class II viral fusion protein structures reveal similar principles in membrane fusion, Mol. Membr. Biol. 21, 361–371 (2004).CrossRefPubMedGoogle Scholar
  6. 6.
    J. S. Peiris, K. Y. Yuen, A. D. Osterhaus, and K. Stohr, The severe acute respiratory syndrome, N. Engl. J. Med. 349, 2431–2441 (2003).CrossRefPubMedGoogle Scholar
  7. 7.
    R. A. Fouchier, N. G. Hartwig, T. M. Bestebroer, B. Niemeyer, J. C. de Jong, J. H. Simon, and A. D. Osterhaus, A previously undescribed coronavirus associated with respiratory disease in humans, Proc. Natl. Acad. Sci. USA 101, 6212–6216 (2004).CrossRefPubMedGoogle Scholar
  8. 8.
    L. van der Hoek, K. Pyrc, M. F. Jebbink, W. Vermeulen-Oost, R. J. Berkhout, K. C. Wolthers, P. M. Wertheim-van Dillen, J. Kaandorp, J. Spaargaren, and B. Berkhout, Identification of a new human coronavirus, Nat. Med. 10, 368–373 (2004).CrossRefPubMedGoogle Scholar
  9. 9.
    W. Li, M. J. Moore, N. Vasilieva, J. Sui, S. K. Wong, M. A. Berne, M. Somasundaran, J. L. Sullivan, K. Luzuriaga, T. C. Greenough, H. Choe, and M. Farzan, Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus, Nature 426, 450–454 (2003).CrossRefPubMedGoogle Scholar
  10. 10.
    H. Hofmann, K. Hattermann, A. Marzi, T. Gramberg, M. Geier, M. Krumbiegel, S. Kuate, K. Uberla, M. Niedrig, and S. Pöhlmann, S protein of severe acute respiratory syndrome-associated coronavirus mediates entry into hepatoma cell lines and is targeted by neutralizing antibodies in infected patients, J. Virol. 78, 6134–6142 (2004).CrossRefPubMedGoogle Scholar
  11. 11.
    J. Gu, E. Gong, B. Zhang, J. Zheng, Z. Gao, Y. Zhong, W. Zou, J. Zhan, S. Wang, Z. Xie, H. Zhuang, B. Wu, H. Zhong, H. Shao, W. Fang, D. Gao, F. Pei, X. Li, Z. He, D. Xu, X. Shi, V. M. Anderson, and A. S. Leong, Multiple organ infection and the pathogenesis of SARS, J. Exp. Med. 202, 415–424 (2005).CrossRefPubMedGoogle Scholar
  12. 12.
    K. Hattermann, M. A. Muller, A. Nitsche, S. Wendt, M. O. Donoso, and M. Niedrig, Susceptibility of different eukaryotic cell lines to SARS-coronavirus, Arch. Virol. 150, 1023–1031 (2005).CrossRefPubMedGoogle Scholar
  13. 13.
    G. Simmons, J. D. Reeves, A. J. Rennekamp, S. M. Amberg, A. J. Piefer, and P. Bates, Characterization of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike glycoprotein-mediated viral entry, Proc. Natl. Acad. Sci. USA 101, 4240–4245 (2004).CrossRefPubMedGoogle Scholar
  14. 14.
    Z. Y. Yang, Y. Huang, L. Ganesh, K. Leung, W. P. Kong, O. Schwartz, K. Subbarao, and G. J. Nabel, pH-dependent entry of severe acute respiratory syndrome coronavirus is mediated by the spike glycoprotein and enhanced by dendritic cell transfer through DC-SIGN, J. Virol. 78, 5642–5650 (2004).CrossRefPubMedGoogle Scholar
  15. 15.
    S. K. Wong, W. Li, M. J. Moore, H. Choe, and M. Farzan, A 193-amino acid fragment of the SARS coronavirus S protein efficiently binds angiotensin-converting enzyme 2, J. Biol. Chem. 279, 3197–3201 (2004).CrossRefPubMedGoogle Scholar
  16. 16.
    H. Hofmann, M. Geier, A. Marzi, M. Krumbiegel, M. Peipp, G. H. Fey, T. Gramberg, and S. Pöhlmann, Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor, Biochem. Biophys. Res. Commun. 319, 1216–1221 (2004).CrossRefPubMedGoogle Scholar
  17. 17.
    E. C. Mossel, C. Huang, K. Narayanan, S. Makino, R. B. Tesh, and C. J. Peters, Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication, J. Virol. 79, 3846–3850 (2005).CrossRefPubMedGoogle Scholar
  18. 18.
    S. Ugolini, I. Mondor, and Q. J. Sattentau, HIV-1 attachment: another look, Trends Microbiol. 7, 144–149 (1999).CrossRefPubMedGoogle Scholar
  19. 19.
    Y. van Kooyk and T. B. Geijtenbeek, DC-SIGN: escape mechanism for pathogens, Nat. Rev. Immunol. 3, 697–709 (2003).CrossRefPubMedGoogle Scholar
  20. 20.
    A. A. Bashirova, T. B. Geijtenbeek, G. C. van Duijnhoven, S. J. van Vliet, J. B. Eilering, M. P. Martin, L. Wu, T. D. Martin, N. Viebig, P. A. Knolle, V. N. KewalRamani, Y. van Kooyk, and M. Carrington, A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection, J. Exp. Med. 193, 671–678 (2001).CrossRefPubMedGoogle Scholar
  21. 21.
    S. Pöhlmann, E. J. Soilleux, F. Baribaud, G. J. Leslie, L. S. Morris, J. Trowsdale, B. Lee, N. Coleman, and R. W. Doms, DC-SIGNR, a DC-SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans, Proc. Natl. Acad. Sci. USA 98, 2670–2675 (2001).CrossRefPubMedGoogle Scholar
  22. 22.
    A. Marzi, T. Gramberg, G. Simmons, P. Moller, A. J. Rennekamp, M. Krumbiegel, M. Geier, J. Eisemann, N. Turza, B. Saunier, A. Steinkasserer, S. Becker, P. Bates, H. Hofmann, and S. Pöhlmann, DC-SIGN and DC-SIGNR interact with the glycoprotein of Marburg virus and the S protein of severe acute respiratory syndrome coronavirus, J. Virol. 78, 12090–12095 (2004).CrossRefPubMedGoogle Scholar
  23. 23.
    S. A. Jeffers, S. M. Tusell, L. Gillim-Ross, E. M. Hemmila, J. E. Achenbach, G. J. Babcock, W. D. Thomas, Jr., L. B. Thackray, M. D. Young, R. J. Mason, D. M. Ambrosino, D. E. Wentworth, J. C. Demartini, and K. V. Holmes, CD209L (L-SIGN) is a receptor for severe acute respiratory syndrome coronavirus, Proc. Natl. Acad. Sci. USA 101, 15748–15753 (2004).CrossRefPubMedGoogle Scholar
  24. 24.
    H. Hofmann, K. Pyrc, H. L. van der, M. Geier, B. Berkhout, and S. Pöhlmann, Human coronavirus NL63 employs the severe acute respiratory syndrome coronavirus receptor for cellular entry, Proc. Natl. Acad. Sci. USA 102, 7988–7993 (2005).CrossRefPubMedGoogle Scholar
  25. 25.
    G. Simmons, D. N. Gosalia, A. J. Rennekamp, J. D. Reeves, S. L. Diamond, and P. Bates, Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry, Proc. Natl. Acad. Sci. USA 102, 11876–11881 (2005).CrossRefPubMedGoogle Scholar
  26. 26.
    K. Kuba, Y. Imai, S. Rao, H. Gao, F. Guo, B. Guan, Y. Huan, P. Yang, Y. Zhang, W. Deng, L. Bao, B. Zhang, G. Liu, Z. Wang, M. Chappell, Y. Liu, D. Zheng, A. Leibbrandt, T. Wada, A. S. Slutsky, D. Liu, C. Qin, C. Jiang, and J. M. Penninger, A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus-induced lung injury, Nat. Med. 11, 875–879 (2005).CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Heike Hofmann
    • 1
  • Andrea Marzi
    • 2
  • Thomas Gramberg
    • 3
  • Martina Geier
    • 4
  • Krzysztof Pyrc
    • 5
  • Lia van der Hoek
    • 6
  • Ben Berkhout
    • 7
  • Stefan Pöhlmann
    • 8
  1. 1.University of Erlangen-NürnbergGermany
  2. 2.University of Erlangen-NürnbergGermany
  3. 3.University of Erlangen-NürnbergGermany
  4. 4.University of Erlangen-NürnbergGermany
  5. 5.University of AmsterdamThe Netherlands
  6. 6.University of AmsterdamThe Netherlands
  7. 7.University of AmsterdamThe Netherlands
  8. 8.University of Erlangen-NürnbergGermany

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