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Decellularization Concept in Regenerative Medicine

  • Özge Sezin SomuncuEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series

Abstract

Decellularized organs and tissues are effectively utilized in a diversity of regenerative medicine purposes, and the decellularization approaches employed differ as broadly as the tissues/organs of concern. Biological scaffold substances formed by extracellular matrix (ECM) are mostly produced with methods that include decellularization of tissues. Conservation of the multifaceted arrangement and three-dimensional (3D) construction of the ECM is very wanted but it is documented that almost every approach of decellularization cause disturbance of the organization and possible forfeiture of surface organization and conformation. The competence of cell elimination from a tissue is reliant on the basis of the tissue and the precise physical, chemical, and enzymatic approaches that are utilized. Here, the most frequently applied and newly developed decellularization techniques are designated, organ engineering with decellularized scaffolds for different organs, recent knowledge in the field are explained.

Keywords

Decellularization Regenerative medicine Recellularization Tissue engineering 

Abbreviation

3D

Three dimensional

ADSCs

Adipose-derived stem cells

CC10

Secretoglobin Family 1A

CCSP

Clara cell secretory protein

CD 31

Cluster of differentiation 31

CK18

Keratin 18

ECM

Extracellular matrix

ESCs

Embryonic stem cells

FOXJ1

Forkhead box protein J1

GAG

Glycosaminoglycans

Mg

Miligram

MIN-6

Mouse insulinoma 6

Ng

Nano gram

Nkx2.1

NK2 Homeobox 1

PBS

Phosphate buffered saline

PDGFR

Platelet derived growth factor receptor

ProSPC

Alveolar type 2 cell marker

SDS

Sodium dodecyl sulfate

SPC

Pulmonary-associated surfactant protein C

TNF

Tumor necrosis factor

TTF-1

Transcription termination factor 1

References

  1. Agmon G, Christman KL (2016) Controlling stem cell behavior with decellularized extracellular matrix scaffolds. Curr Opin Solid State Mater Sci 20(4):193–201Google Scholar
  2. Badylak SF, Taylor D, Uygun K (2011) Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng 13:27–53Google Scholar
  3. Balestrini JL et al (2015) Production of decellularized porcine lung scaffolds for use in tissue engineering. Integr Biol 7(12):1598–1610Google Scholar
  4. Bourgine PE et al (2013) Tissue decellularization by activation of programmed cell death. Biomaterials 34(26):6099–6108Google Scholar
  5. Clevers H (2015) What is an adult stem cell? Science 350(6266):1319–1320Google Scholar
  6. Conrad C et al (2010) Bio-engineered endocrine pancreas based on decellularized pancreatic matrix and mesenchymal stem cell/islet cell coculture. J Am Coll Surg 211(3):S62–S62Google Scholar
  7. Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32(12):3233–3243Google Scholar
  8. Duisit J et al (2018) Perfusion-decellularization of human ear grafts enables ECM-based scaffolds for auricular vascularized composite tissue engineering. Acta Biomater 73:339–354Google Scholar
  9. Frohlich M et al (2010) Bone grafts engineered from human adipose-derived stem cells in perfusion bioreactor culture. Tissue Eng A 16(1):179–189Google Scholar
  10. Garreta E et al (2017) Tissue engineering by decellularization and 3D bioprinting. Mater Today 20(4):166–178Google Scholar
  11. Gilpin A, Yang Y (2017) Decellularization strategies for regenerative medicine: from processing techniques to applications. Biomed Res Int 2017:1–13Google Scholar
  12. Gray FL et al (2012) Prenatal tracheal reconstruction with a hybrid amniotic mesenchymal stem cells-engineered construct derived from decellularized airway. J Pediatr Surg 47(6):1072–1079Google Scholar
  13. Hassanein W et al (2017) Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold. Organogenesis 13(1):16–27Google Scholar
  14. Heidenreich PA et al (2011) Forecasting the future of cardiovascular disease in the United States a policy statement from the American Heart Association. Circulation 123(8):933–944Google Scholar
  15. Hopkins RA et al (2013) Bioengineered human and allogeneic pulmonary valve conduits chronically implanted orthotopically in baboons: hemodynamic performance and immunologic consequences. J Thorac Cardiovasc Surg 145(4):1098Google Scholar
  16. Hoshiba T et al (2010) Decellularized matrices for tissue engineering. Expert Opin Biol Ther 10(12):1717–1728Google Scholar
  17. Hung SH et al (2016) Preliminary experiences in trachea scaffold tissue engineering with segmental organ decellularization. Laryngoscope 126(11):2520–2527Google Scholar
  18. Jansen J et al (2014) Biotechnological challenges of bioartificial kidney engineering. Biotechnol Adv 32(7):1317–1327Google Scholar
  19. Kang HJ et al (2014) In vivo cartilage repair using adipose-derived stem cell-loaded decellularized cartilage ECM scaffolds. J Tissue Eng Regen Med 8(6):442–453Google Scholar
  20. Khan AA et al (2014) Repopulation of decellularized whole organ scaffold using stem cells: an emerging technology for the development of neo-organ. J Artif Organs 17(4):291–300Google Scholar
  21. Laronda MM et al (2015) Initiation of puberty in mice following decellularized ovary transplant. Biomaterials 50:20–29Google Scholar
  22. Lu TY et al (2013) Repopulation of decellularized mouse heart with human induced pluripotent stem cell-derived cardiovascular progenitor cells. Nat Commun 4:2307Google Scholar
  23. Mao AS, Mooney DJ (2015) Regenerative medicine: current therapies and future directions. Proc Natl Acad Sci U S A 112(47):14452–14459Google Scholar
  24. Meng FW et al (2017) Whole liver engineering: a promising approach to develop functional liver surrogates. Liver Int 37(12):1759–1772Google Scholar
  25. Ng SLJ et al (2011) Lineage restricted progenitors for the repopulation of decellularized heart. Biomaterials 32(30):7571–7580Google Scholar
  26. Ott HC et al (2008) Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart. Nat Med 14(2):213–221Google Scholar
  27. Papadimitropoulos A et al (2015) Engineered decellularized matrices to instruct bone regeneration processes. Bone 70:66–72Google Scholar
  28. Parmaksiz M et al (2016) Clinical applications of decellularized extracellular matrices for tissue engineering and regenerative medicine. Biomed Mater 11(2):022003Google Scholar
  29. Rana D et al (2017) Development of decellularized scaffolds for stem cell-driven tissue engineering. J Tissue Eng Regen Med 11(4):942–965Google Scholar
  30. Rijal G (2017) The decellularized extracellular matrix in regenerative medicine. Regen Med 12(5):475–477Google Scholar
  31. Salvatori M et al (2014) Extracellular matrix scaffold technology for bioartificial pancreas engineering: state of the art and future challenges. J Diabetes Sci Technol 8(1):159–169Google Scholar
  32. Seetapun D, Ross JJ (2017) Eliminating the organ transplant waiting list: the future with perfusion decellularized organs. Surgery 161(6):1474–1478Google Scholar
  33. Song JJ, Ott HC (2011) Organ engineering based on decellularized matrix scaffolds. Trends Mol Med 17(8):424–432Google Scholar
  34. Tapias LF, Ott HC (2014) Decellularized scaffolds as a platform for bioengineered organs. Curr Opin Organ Transplant 19(2):145–152Google Scholar
  35. Taylor DA et al (2018) Decellularized matrices in regenerative medicine. Acta Biomater 74:74–89Google Scholar
  36. Uygun BE et al (2010) Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nat Med 16(7):814–U120Google Scholar
  37. Wagner DE et al (2013) Can stem cells be used to generate new lungs? Ex vivo lung bioengineering with decellularized whole lung scaffolds. Respirology 18(6):895–911Google Scholar
  38. Wang B et al (2010) Fabrication of cardiac patch with decellularized porcine myocardial scaffold and bone marrow mononuclear cells. J Biomed Mater Res A 94a(4):1100–1110Google Scholar
  39. Wu D et al (2015) 3D culture of min-6 cells on decellularized pancreatic scaffold: in vitro and in vivo study. Biomed Res Int 2015:432645Google Scholar
  40. Yu YL et al (2016) Decellularized scaffolds in regenerative medicine. Oncotarget 7(36):58671–58683Google Scholar
  41. Zang MQ et al (2013) Decellularized tracheal matrix scaffold for tracheal tissue engineering: in vivo host response. Plast Reconstr Surg 132(4):549e–559eGoogle Scholar
  42. Zia S et al (2016) Hearts beating through decellularized scaffolds: whole-organ engineering for cardiac regeneration and transplantation. Crit Rev Biotechnol 36(4):705–715Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Medical BiologyBahçeşehir University Faculty of MedicineİstanbulTurkey

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