Challenges in Bio-fabrication of Organoid Cultures

  • Weijie Peng
  • Pallab Datta
  • Yang Wu
  • Madhuri Dey
  • Bugra Ayan
  • Amer Dababneh
  • Ibrahim T. OzbolatEmail author
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1107)


Three-dimensional (3D) organoids have shown advantages in cell culture over traditional two-dimensional (2D) culture, and have great potential in various applications of tissue engineering. However, there are limitations in current organoid fabrication technologies, such as uncontrolled size, poor reproductively, and inadequate complexity of organoids. In this chapter, we present the existing techniques and discuss the major challenges for 3D organoid biofabrication. Future perspectives on organoid bioprinting are also discussed, where bioprinting technologies are expected to make a major contribution in organoid fabrication, such as realizing mass production and constructing complex heterotypic tissues, and thus further advance the translational application of organoids in tissue engineering and regenerative medicine as well drug testing and pharmaceutics.


3D culture Bioprinting Organoids Regenerative medicine Tissue engineering 







Adipose-derived mesenchymal stem cells


adipose-derived stem cell


biological laser printing


CXC ligand


CXC receptor


droplet-based bioprinting


dental pulp cells


extrusion-based bioprinting


embryonic stem


hyaluronic acid


human epidermal growth receptor


hepatocyte growth factor


hypoxia-inducible factor


hydrogel tissue constructs


human umbilical vein endothelial cells


laser-based bioprinting


mitogen activate protein kinase


matrix assisted pulsed laser evaporation-direct write


multicellular spheroids


mesenchymal stem cells


poly (2-hydroxethyl methacrylate)


phosphoinositide 3-kinase


poly (N-isopropylacrylamide)


polyvinyl alcohol


Rat embryo fibroblasts


arginylglycylaspartic acid


stromal cell-derived factor


superparamagnetic iron oxide nanoparticles


tissue culture dish


tissue engineering


tumor necrosis factor


vascular endothelial growth factor



This work has been supported by National Science Foundation Awards # 1624515, National Institutes of Health Grant #R21 CA224422-01A1, an ENGINE grant from Penn State, Diabetes in Action Research and Education Foundation grant # 426, a Wells Fargo grant, the China Scholarship Council 201308360128 and the Oversea Sailing Project from Jiangxi Association for Science and Technology. The authors also acknowledge Indian Council of Medical Research, Government of India, for financial assistance to P.D. The authors are grateful to the support from the Turkish Ministry of National Education for providing graduate scholarship to B.A.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Weijie Peng
    • 1
    • 2
    • 3
  • Pallab Datta
    • 4
  • Yang Wu
    • 3
    • 5
  • Madhuri Dey
    • 5
    • 6
  • Bugra Ayan
    • 3
    • 5
  • Amer Dababneh
    • 7
  • Ibrahim T. Ozbolat
    • 3
    • 5
    • 8
    • 9
    Email author
  1. 1.Jiangxi Academy of Medical ScienceHospital of Nanchang UniversityNanchangChina
  2. 2.Department of PharmacologyNanchang UniversityNanchangChina
  3. 3.Engineering Science and Mechanics DepartmentPenn State UniversityUniversity ParkUSA
  4. 4.Centre for Healthcare Science and TechnologyIndian Institute of Engineering Science and Technology ShibpurHowrahIndia
  5. 5.The Huck Institutes of the Life SciencesPenn State UniversityUniversity ParkUSA
  6. 6.Department of ChemistryPenn State UniversityUniversity ParkUSA
  7. 7.Center for Computer-Aided Design, College of EngineeringUniversity of IowaIowa CityUSA
  8. 8.Biomedical Engineering DepartmentPenn State UniversityUniversity ParkUSA
  9. 9.Materials Research InstitutePenn State UniversityUniversity ParkUSA

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