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Anti-cancer Effect of Xao Tam Phan Paramignya trimera Methanol Root Extract on Human Breast Cancer Cell Line MCF-7 in 3D Model

  • Lam-Huyen Nguyen-Thi
  • Sinh Truong Nguyen
  • Thao Phuong Tran
  • Chinh-Nhan Phan-Lu
  • Phuc Van PhamEmail author
  • Trung The Van
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1292)

Abstract

Background: Cancer is one of the leading causes of death in the world. A great deal of effort has been made to discover new agents for cancer treatment. Xao tam phan (Paramignya trimera) is a traditional medicine of Vietnam used in cancer treatment for a long time, yet there is not much scientific evidence proving its anticancer potency. The study aimed to evaluate the toxicity of Paramignya trimera extract (PTE) on multicellular tumor spheres (MCTS) of MCF-7 cells using hanging drop technique. Methods: Firstly, MCF-7 cells were seeded on hanging drop plates, spheroid size was tracked, and growth curve was measured by MTT assay and AlamarBlue® assay. The necrotic core of MCTS was evaluated by propidium iodide (PI) staining. Toxicity of doxorubicin (DOX) and tirapazamine (TPZ) was then tested on 3D model compared to 2D culture condition. Results: The results showed that the IC50 of DOX on 3D MCF-7 cells was nearly 50 times greater than monolayer MCF-7 cells. In contrast, TPZ (an agent which is specifically toxic under hypoxic conditions) had significantly lower IC50 in 3D condition than in 2D. The toxicity tests for PTE showed that PTE strongly inhibited MCF-7 cells in both 2D and 3D conditions. Interestingly, the IC50 of PTE in 3D model was remarkably lower than in 2D (IC50 value was 168.9 ± 11.65 μg/ml compared to 260.8 ± 16.54 μg/ml, respectively). The invasion assay showed that PTE completely inhibited invasion of MCF-7 cells at 250 μg/mL concentration. Also, flow cytometry results indicated that PTE effectively induced apoptosis in MCF-7 spheroids in 3D condition at 250 μg/mL concentration. Conclusion: The results from this study emphasize the promise of PTE in cancer therapy.

Keywords

3D tumor sphere Anticancer Apoptosis Extract MCF-7 Multicellular tumor spheres Paramignya trimera Tirapazamine Toxicity TPZ Xao tam phan 

Abbreviations

DOX

Doxorubicin

ECM

Extracellular matrix

FBS

Fetal bovine serum

MCTS

Multicellular tumor spheres

PTE

Paramignya trimera extract

PI

Propidium iodide

TPZ

Tirapazamine

Notes

Funding and Grants

This research was funded by Vietnam National University, Ho Chi Minh city, Viet Nam under grant number A2015-18-01/HD-KHCN.

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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • Lam-Huyen Nguyen-Thi
    • 1
    • 2
  • Sinh Truong Nguyen
    • 1
    • 2
  • Thao Phuong Tran
    • 1
  • Chinh-Nhan Phan-Lu
    • 1
    • 2
  • Phuc Van Pham
    • 1
    • 2
    • 3
    Email author
  • Trung The Van
    • 4
  1. 1.Stem Cell InstituteUniversity of Science, VNUHCMHo Chi Minh CityVietnam
  2. 2.Laboratory of Cancer ResearchUniversity of Science, VNUHCMHo Chi Minh CityVietnam
  3. 3.Laboratory of Stem Cell Research and ApplicationUniversity of Science, VNUHCMHo Chi Minh CityVietnam
  4. 4.Ho Chi Minh City Medicine and Pharmacy University, Hospital of Dermatology – Ho Chi Minh CityHo Chi Minh CityVietnam

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