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In Vitro Production of Cartilage Tissue from Rabbit Bone Marrow-Derived Mesenchymal Stem Cells and Polycaprolactone Scaffold

  • Thuy Thi-Thanh Dao
  • Ngoc Bich VuEmail author
  • Liem Hieu Pham
  • Long Van Gia
  • Ha Thi-Ngan Le
  • Lan Thi Phi
  • Khanh Hong-Thien Bui
  • Phuong Thi-Bich Le
  • Phuc Van Pham
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1084)

Abstract

In vitro production of tissues or tissue engineering is a promising approach to produce artificial tissues for regenerative medicine. There are at least three important components of tissue engineering, including stem cells, scaffolds and growth factors. This study aimed to produce cartilage tissues in vitro from culture and chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (BMMSCs), induced by chondrogenesis medium, on biodegradable polycaprolactone (PCL) scaffolds. BMMSCs were isolated from rabbit bone marrow according to the standard protocol. The adherence, proliferation and differentiation of BMMSCs on scaffolds were investigated using two scaffold systems: PCL scaffolds and collagen-coated PCL (PCL/col) scaffolds. The results showed that BMMSCs could attach and grow on both PCL and PCL/col scaffolds. However, the adhesion efficacy of BMMSCs on the PCL/col scaffolds was significantly better than on PCL scaffolds. Under induced conditions, BMMSCs on PLC/col scaffolds showed increased aggrecan accumulation and upregulated expression of chondrogenesis-associated genes (e.g. collagen type II, collagen type I, aggrecan and collagen type X) after 3, 7, 21 and 28 days of induction. These in vitro cartilage tissues could form mature chondrocyte-like cells after they were grafted into rabbits. The results suggest that use of BMMSCs in combination with polycaprolactone scaffolds and chondrogenesis medium can be a way to form in vitro cartilage tissue.

Keywords

Cartilage tissue engineering Engineered cartilage Polycaprolactone scaffolds Regenerative medicine Tissue engineering 

Abbreviations

BMMSCs

Bone marrow-derived mesenchymal stem cells

CD

Cluster of differentiation

ECM

Extracellular matrix

GFP

Green fluorescent protein

MSC

Mesenchymal stem cell

PBS

Phosphate buffer saline

PCL

Polycaprolactone

PCL/col

Collagen-coated PCL

PCR

Polymer chain reaction

Notes

Acknowledgements

This research is funded by Vietnam National University Ho Chi Minh City (VNU-HCM) under grant numbers TX2017-18-02 and C2017-18-24/HĐ-KHCN and University of Science Ho Chi Minh City under grant number T2017-44.

Conflict of Interest

The authors declare that there is no conflict of interest regarding the publication of this paper.

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Thuy Thi-Thanh Dao
    • 1
    • 2
  • Ngoc Bich Vu
    • 1
    • 2
    Email author
  • Liem Hieu Pham
    • 3
  • Long Van Gia
    • 4
  • Ha Thi-Ngan Le
    • 1
    • 2
  • Lan Thi Phi
    • 1
    • 2
  • Khanh Hong-Thien Bui
    • 5
  • Phuong Thi-Bich Le
    • 6
  • Phuc Van Pham
    • 1
    • 2
  1. 1.Stem Cell InstituteUniversity of Science, VNUHCMHo Chi Minh CityVietnam
  2. 2.Laboratory of Stem Cell Research and ApplicationUniversity of Science, VNUHCMHo Chi Minh CityVietnam
  3. 3.Pham Ngoc Thach University of MedicineHo Chi Minh cityVietnam
  4. 4.Institute of Applied Mechanics and Informatics, Vietnam Academy of Science and TechnologyHo Chi Minh cityVietnam
  5. 5.University of Medical CenterHo Chi Minh University of Medicine and PharmacyHo Chi Minh CityVietnam
  6. 6.Stem Cell Unit, Van Hanh HospitalHo Chi Minh cityVietnam

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