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The Role of the Pyranopterin Dithiolene Component of Moco in Molybdoenzyme Catalysis

  • Sharon J. Nieter BurgmayerEmail author
  • Martin L. KirkEmail author
Chapter
Part of the Structure and Bonding book series (STRUCTURE, volume 179)

Abstract

An overview of the pyranopterin dithiolene (MPT) component of the molybdenum cofactor (Moco) and how MPT may contribute to enzymatic catalysis is presented. The chapter begins with a brief review of MPT and Moco biosynthesis and continues to explore the nature of what is arguably the most electronically complex ligand in biology. To explore this complexity, we have dissected MPT into its relevant molecular components. These include the redox-active ene-1,2-dithiolate (dithiolene) and pterin moieties, which are bridged by a pyran that may be found in ring-opened or ring-closed configurations. The various redox possibilities of MPT bound to Mo are presented, along with the electronic structure of the redox components. MPTs are found to display a remarkable conformational variance in pyranopterin Mo enzymes. This is discussed in terms of a relationship to enzyme function and the potential for the observed non-planer distortions to reflect different MPT oxidation and tautomeric states. The chapter ends with a series of case studies featuring model compounds that highlight how biomimetic small molecule studies have contributed to furthering our understanding of the roles this remarkable ligand plays in the catalytic cycles of the enzymes.

Keywords

Dithiolene Moco Molybdenum cofactor Molybdenum enzymes Molybdopterin Pyranopterin 

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of ChemistryBryn Mawr CollegeBryn MawrUSA
  2. 2.Department of Chemistry and Chemical BiologyThe University of New MexicoAlbuquerqueUSA

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