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New Trends in Fluorescent Reporters in Biology
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Abstract
Fluorescent dyes play a prominent role in the detection and quantification of components in biological samples. Analytical methods based on fluorescent dyes include fluorescence microscopy, fluorescent immunoassays, nucleic acid assays, flow cytometry, and fluorescent imaging. During the past decade, most research effort by synthetic chemistry companies focused on three different areas: (a) improvement of existing cyanine dyes, (b) development of new dyes with large Stokes-shifts (LSS), and (c) development of dye assemblies, namely, fluorescent conjugated polymers (FCP) and fluorescent dye-doped silica nanoparticles (FSNP).
Keywords
4,4-Difluoro-4-bora-3a,4a-diaza-indacene (BODIPY) Cyanines Flow cytometry Fluorescent conjugated polymers (FCP) Fluorescent dye-doped silica nanoparticles (FSNP) Förster resonance energy transfer (FRET) InGaN (indium gallium nitride) InGaP (indium gallium phosphide) Large stokes-shift (LSS) dyes Laser diode excitation Major histocompatibility complex (MHC) Multicolor panels Near infrared (NIR) Plasma protein binding (PPB)References
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