Advertisement

Molecular Differential Diagnoses of Infectious Diseases: Is the Future Now?

  • Jian Han
Chapter

Abstract

The clinical presentations for most infectious agents are often not specific enough to allow for a definitive diagnosis. Coughing and fever, for example, are symptoms that may be caused by many different bacterial or viral infections. Thus, for better treatment and disease control, a molecular differential diagnostic (MDD) assay that can identify, differentiate, and pinpoint the offending pathogen associated with a clinical syndrome (Fig. 27.1) is needed. MDDs are essential tools for effective infectious disease surveillance, biodefense, and personalized medicine.

Keywords

West Nile Virus Capture Probe Ligase Chain Reaction American Type Culture Collection Strain Luminex xMAP 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Adams, C. & Brantner, V. V. (2004). Estimating the costs of new drug development: is it really $802 m? Social Science Research Network. http://papers.ssrn.com/sol3/papers.cfm? abstract id=640563 Google Scholar
  2. Dean, A., Lamson, D. M., Han, J., et al. (2005). Evaluation of Genaco’s Molecular Differential Diagnostic technology for the detection of RNA respiratory viruses. Abstract #67663. 22nd Annual Clinical Virology Symposium and Annual Meeting of the Pan American Society for Clinical Virology. Google Scholar
  3. Dunbar, S. A., Vander Zee, C. A., Oliver, K. G., Karem, K. L., & Jacobson, J. W. (2003). Quantitative, multiplexed detection of bacterial pathogens: DNA and protein applications of the Luminex LabMAP system. J Microbiol Methods, 53, 245–252. PubMedCrossRefGoogle Scholar
  4. Farr, B. M. & Bellingan, G. (2004). Pro/con clinical debate: isolation precautions for all intensive care unit patients with methicillin-resistant Staphylococcus aureus colonization are essential. Crit Care, 8(3), 153–156. PubMedCrossRefGoogle Scholar
  5. Farr, B. M., Salgado, C. D., Karchmer, T. B., & Sherertz, R. (2001). Can antibiotic-resistant nosocomial infections be controlled? Lancet Infect Dis, 1, 38–45. CrossRefGoogle Scholar
  6. Fields, B. S., et al. (2005) Collaborative development of a multiplex assay system for respiratory infections. Personal communications. Google Scholar
  7. Francois, P., Renzi, G., Pittet, D., et al. (2004). A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. J Clin Microbiol, 42(7), 3309–3312 PubMedCrossRefGoogle Scholar
  8. Gröndahl, B., Puppe, W., Hoppe, A., Kühne, I., Josef A. I., Weigl, J. A. I., & Schmitt, H-J. (1999). Rapid identification of nine microorganisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study. J Clin Microbiol, 37(1), 1–7. PubMedGoogle Scholar
  9. Hallin, M., Maes, N., Byl, B., Jacobes, F., De Gheldre,Y., & Struelens, M. J. (2003). Clinical impact of a PCR assay for identification of Staphylococcus aureus and determination of methicillin resistance directly from blood cultures. J Clin Microbiol, 41(8)3942–3944. PubMedCrossRefGoogle Scholar
  10. Heffler, S., Smith, S., and Keehan, S., et al. (2004). Health spending projections through 2013. Health AffairsWeb Exclusive, W4-79 through W4-93. http://content.healthaffairs.org/cgi/content/full/hlthaff. W4.79v1/DC1 Google Scholar
  11. Hindiyeh, M., Hillyard D. R., & Carroll, K. C. (2001). Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens. Am J Clin Pathol, 116(2), 218–224. PubMedCrossRefGoogle Scholar
  12. Kornegay, J. R., Shepard, A. P., Hankins, C., et al. (2001). Nonisotopic detection of human papillomavirus DNA in clinical specimens using a consensus PCR and a generic probe mix in an enzyme-linked immunosorbent assay format. J Clin Microbiol, 39(10), 3530–3536. PubMedCrossRefGoogle Scholar
  13. Louie, L., Goodfellow, J., Mathieu, P., et al. (2002). Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay. J Clin Microbiol, 40(8), 2786–2790. PubMedCrossRefGoogle Scholar
  14. Ma, X., Han, J., Wang, Z. H., et al. (2004). SARS Differential Diagnosis with a Bead Array Method. Reiner, J. Zhao, K., Chen, S. H., Guo, M. eds. Frontiers of Biotechnology& Pharmaceuticals. Volume Four. Science Press, New York, Vol. 4. pp. 1–29. Google Scholar
  15. Reiner, J., Zhao, K., Chen, S. H., & Guo, M. eds. (2004). Frontiers of Biotechnology& Pharmaceuticals, vol. four. Science Press, New York, pp. 1–29. Google Scholar
  16. Sakai, H., Procop, G. W., Kobayashi, N., et al. (2004). Simultaneous detection of Staphylococcus aureus and coagulase-negative staphylococci in positive blood cultures by realtime PCR with two fluorescence resonance energy transfer probe sets. J. Clin Microbiol, 42(12), 5739–5744. PubMedCrossRefGoogle Scholar
  17. Strommenger, B., Kettlitz, C., Werner, G., & Witte, W. (2003). Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus. J. Clin Microbiol, 41(9), 4089–4094. PubMedCrossRefGoogle Scholar
  18. Swan, D., Tucker, R. A., & Unger, E. R. (2005). Evaluation of Genaco HPV genotyping assay system. Personal communications. Google Scholar
  19. Wallace, J., Woda, B. A., & Pihan, G. (2005). Facile, comprehensive, high-throughput genotyping of human genital papillomaviruses using spectrally addressable liquid bead microarrays. J Mol Diagn, 7(1), 72–80. PubMedGoogle Scholar
  20. Wichelhaus, T. A., Böddinghaus, B., Besier, S., et al. (2002). Biological cost of rifampin resistance from the perspective of Staphylococcus aureus. Antimicrob Agents Chemother, 46(11), 3381–3385. CrossRefGoogle Scholar
  21. Yoshikawa, H., Kawana, T., Kitagawa, K., Mizuno, M., Yoshikura, H., & Iwamoto, A. (1991). Detection and typing of multiple genital human papillomaviruses by DNA amplification with consensus primers. Jpn J Cancer Res, 82(5), 524–531. PubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Jian Han

There are no affiliations available

Personalised recommendations