EPR Spectroscopic Ruler: the Method and its Applications
The distance measurement method based on Fourier deconvolution of dipolar coupling in spin-labeled EPR spectra provides a new way of examining the structure and function of biological macromolecules. In this chapter, we describe a new approach that has been developed for effective and reasonably accurate data analysis, followed by discussions of several successful applications to interesting biological problems on membrane-associated proteins. This method of EPR spectroscopic ruler has emerged as a powerful tool to investigate the functions of membrane-associated proteins.
KeywordsSpin Label Coiled Coil Snare Complex Interspin Distance Distance Measurement Method
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